Core E: Omics Core

核心 E：组学核心

• 批准号: 10454256
• 负责人: Sid E O'Bryant
• 金额: $ 312.05万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10454256
• 关键词: Alleles; Alzheimer' s Disease; Alzheimer’s disease biomarker; Amyloid beta-42; Autopsy; Bioinformatics; Biological Assay; Biological Markers; Biometry; Blood; Central Nervous System Diseases; Cerebrospinal Fluid; Clinical Protocols; Collection; Communities; Complement; DNA; Data; Data Set; Databases; Development; Diagnosis; Diagnostic; Disease; Down Syndrome; Enzyme-Linked Immunosorbent Assay; Fatty Acids; Generations; Genetic; Genome; Genomics; Glycolysis; IL6 gene; Laboratories; Leukocytes; Liquid substance; Mass Spectrum Analysis; Measures; Methods; Minor; Modality; Modeling; Molecular Weight; National Institute on Aging; Oxidative Phosphorylation; Participant; Pathway interactions; Physiological Processes; Plasma; Prognosis; Proteins; Proteomics; Protocols documentation; Quality Control; Research Personnel; Research Project Grants; Risk; S-nitro-N-acetylpenicillamine; Sampling; Scientist; Serum; Shotguns; Source; Specificity; System; Systems Biology; TNF gene; Technology; Therapeutic; United States National Institutes of Health; Universities; Validation; Variant; Visit; Washington; Work; alpha synuclein; base; biobank; biomarker discovery; circulating biomarkers; data integration; data management; data storage site; epigenomics; follow-up; genome wide association study; lipid metabolism; metabolome; metabolomics; neurogranin; neuroimaging; novel; novel marker; oxidation; peripheral blood; polygenic risk score; prognostic; protein biomarkers; repository; single molecule; tau Proteins; transcriptome sequencing; transcriptomics; translational applications; whole genome

Omics Core Abstract The Omics Core (Core E), led by Drs. Sid O’Bryant and Mark Mapstone will use advanced methods to conduct state of the art quantitation of fluid-based biomarkers representing all levels of systems biology; from the genome to the metabolome for use in the proposed projects and to share with the broader scientific community. We will derive genomic, transcriptomic, proteomic, and metabolomic information from peripheral blood and cerebrospinal fluid (CSF) collected from our participants as part of our established clinical protocol. Peripheral blood is an accessible matrix with only minor risks associated with collection and would be preferred for translational applications, while CSF is a CNS-specific matrix with high specificity for CNS disorders like AD in DS. We will employ targeted approaches to quantify known markers from these matrices based on our preliminary work and we will use untargeted methods to quantify a variety of other potential markers. This combined approach will provide the highest likelihood of capturing the most valid and reliable fluid-based biomarkers and a rich dataset for new biomarker discovery by our group and other qualified groups around the world. The Core is represented by world class scientists with expertise in genomics and transcriptomics (Lee, Kamboh, Feingold, Cruchaga, Harari), epigenomics (Tycko), proteomics (O’Bryant, Fagan), and metabolomics (Mapstone, Cheema, Macciardi).

OMICS核心摘要 由Drs领导的Omics Core（核心E）。 Sid O’Bryant和Mark Mapstone将使用高级方法进行 基于流体的生物标志物的最先进的状态代表了所有级别的系统生物学；来自基因组 向代谢组中用于拟议项目，并与更广泛的科学界分享。我们将 从外周血和 作为我们既定的临床方案的一部分，从参与者那里收集的脑脊液（CSF）。外围 血液是一种可访问的矩阵 翻译应用，而CSF是CNS特异性矩阵，具有高特异性的CNS疾病，例如AD DS。我们将采用有针对性的方法来量化这些物品的已知标记 初步工作，我们将使用未靶向的方法来量化各种其他潜在标记。这 组合方法将提供捕获最有效和可靠的基于液体的最高可能性 生物标志物和我们小组和其他合格小组围绕新生物标志物发现的丰富数据集 世界。核心由具有基因组学和转录组学专业知识的世界一流科学家代表（Lee， Kamboh，Feingold，Cruchaga，Harari），表观基因组学（Tycko），蛋白质组学（O'Bryant，Fagan）和代谢组学 （Mapstone，Cheema，Macciardi）。