Shared High-resolution Laser Scanning Microscope with Airyscan 2

与 Airyscan 2 共享高分辨率激光扫描显微镜

• 批准号: 10430985
• 负责人: Chinfei Chen
• 金额: $ 47.65万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10430985
• 关键词: Applications Grants; Architecture; Area; Basic Science; Boston; Brain; Cell Communication; Cells; Core Facility; Development; Funding; Intellectual and Developmental Disabilities Research Centers; Laboratories; Lasers; Microscope; Microscopy; Neurobiology; Neurodegenerative Disorders; Neurodevelopmental Disorder; Noise; Organelles; Pediatric Hospitals; Process; Proteins; Request for Applications; Research; Research Personnel; Resolution; Scanning; Signal Transduction; Speed; System; Tissues; Translational Research; United States National Institutes of Health; axon regeneration; cellular imaging; detector; high throughput screening; instrument; medical schools; neuronal survival; programs; research study; translational applications

ABSTRACT This grant application requests funding support for the purchase of a shared Confocal Microscope System with a high-resolution detector to be installed in the Intellectual and Developmental Disabilities Research Center Cellular Imaging Core, a shared microscopy facility at Boston Children’s Hospital (BCH). The requested instrument combines enhancements in high signal-to-noise ratio, fast acquisition speed and enhanced resolution that are not achievable by other instruments available in the area. These features will benefit a growing number of NIH-funded research studies at BCH and Harvard Medical School that require sub-cellular localization of proteins in fixed and living tissue at high acquisition speeds. Other research programs that need high-resolution & high-throughput screening for translational applications will benefit immensely from this instrument. After installation in the Cellular Imaging Core in the Department of Neurobiology and F.M. Kirby Neurobiology Center at BCH, this state-of-the-art microscope will benefit the 84 Harvard Medical School area laboratories that use the core facility for basic and translational research. Primary Investigators from these labs will use the system to study a wide variety of pressing questions regarding organelle mobility and dynamics, subcellular architecture, protein-protein and cell-cell interactions, and the relations of these basic processes in normal brain development, in axon regeneration and neuronal survival, and in neurodevelopmental and neurodegenerative disorders.

抽象的 本赠款申请要求使用 智力和发育障碍研究中心安装的高分辨率探测器 蜂窝成像核心，波士顿儿童医院（BCH）的共享显微镜设施。请求 仪器组合以高信噪比的增强，快速获取速度和增强 该地区可用的其他工具无法实现的解决方案。这些功能将使 BCH和哈佛医学院的NIH资助研究越来越多，需要亚细胞 蛋白质以高采集速度的固定组织和活组织的定位。其他需要的研究计划 转化应用的高分辨率和高通量筛选将受益于此 乐器。 在神经生物学系和F.M.柯比神经生物学 BCH中心，这款最先进的显微镜将使84个哈佛医学院地区实验室受益 那将核心设施用于基础和翻译研究。这些实验室的主要调查人员将使用 研究有关细胞器迁移率和动态的各种迫切问题的系统 建筑，蛋白质 - 蛋白质和细胞细胞相互作用，以及这些基本过程在正常中的关系 大脑发育，轴突再生和神经元生存期以及神经发育和 神经退行性疾病。