Shared multi-photon confocal microscope

共享多光子共焦显微镜

• 批准号: 8448369
• 负责人: Chinfei Chen
• 金额: $ 49.47万
• 依托单位: BOSTON CHILDREN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-15 至 2014-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8448369
• 关键词: Applications Grants; Area; Biology; Boston; Cell Proliferation; Cell physiology; Communities; Core Facility; Defect; Funding; Image; Imagery; In Situ; Laboratory Research; Life; Mental Retardation and Developmental Disabilities Research Centers; Microscope; Neurobiology; Neuroglia; Neurology; Neurons; Neurosciences; Pain; Pediatric Hospitals; Process; Request for Applications; Research; Research Personnel; Resolution; Scientist; Signal Transduction; Stem cells; Structure; Synaptic plasticity; System; Systems Development; Technology; Tissues; United States National Institutes of Health; axon guidance; body system; cellular imaging; human disease; instrument; intellectual and developmental disability; medical schools; multi-photon; programs; relating to nervous system; research study; sensory system; tissue fixing

DESCRIPTION (provided by applicant): This grant application requests funding for the purchase of a shared Multiphoton Confocal Microscope System for the Intellectual and Developmental Disabilities Research Center (IDDRC) Imaging Core at Children's Hospital, Boston. This microscope offers state-of-the-art technology that will allow many investigators to visualize fluorescent structures deep into living and fixed tissues at unprecedented spatial resolution and with high sensitivity. These features of the multiphoton confocal system will benefit the growing number of NIH- funded research studies at Children's Hospital and the Harvard Medical School Area that require the imaging of cellular processes deep in live tissue. Research programs that require visualization of fine processes of neuronal networks in situ will also profit strongly from access to this instrument. The modern multiphoton confocal system will be incorporated into the Cellular Imaging Core Facility located in the Department of Neurology and F.M. Kirby Neurobiology Center at Children's Hospital. This facility has been operational for over 34 years and serves more than 74 research laboratories at Children's Hospital and throughout Harvard Medical School. Scientists and clinicians in this research community will be able to use this microscope to study a wide variety of problems in neuroscience and other fields of biology, including how the mechanisms of synaptic plasticity, neural-glia interactions, axon guidance defects, pain, stem cell proliferation, sensory system signaling, and organ system development relate to human disease.

描述（由申请人提供）：本赠款申请要求为波士顿儿童医院的智力和发育障碍研究中心（IDDRC）研究中心（IDDRC）成像核心购买共享的多人共聚焦显微镜系统。该显微镜提供了最先进的技术，该技术将使许多研究人员以前所未有的空间分辨率和高灵敏度可视化荧光结构深入生命和固定组织。多光子共聚焦系统的这些特征将使儿童医院和哈佛医学院地区的NIH资助研究越来越多，这些研究需要对活组织深处的细胞过程进行成像。需要可视化神经元网络的精细过程的研究计划也将从访问该仪器的访问中获得强大的利润。现代多光共聚焦系统将被合并到位于神经病学和F.M.的蜂窝成像核心设施中。儿童医院的柯比神经生物学中心。该设施已经运作了34年以上，并在儿童医院和整个哈佛医学院为74多家研究实验室提供服务。该研究界的科学家和临床医生将能够使用该显微镜研究神经科学和其他生物学领域的各种问题，包括突触可塑性，神经 - 网络相互作用的机制如何，轴突指导缺陷，疼痛，干细胞增殖，感觉系统信号和机构系统发展与人类病有关。