Flow Cytometry

流式细胞术

• 批准号: 10400710
• 负责人: Aniruddha J. Deshpande
• 金额: $ 21.23万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10400710
• 关键词: Antibodies; Cancer Center; Cancer Center Support Grant; Cell Separation; Cells; Color; Communication; Computer software; Contract Services; Core Facility; Cytometry; Data Analyses; Development; Doctor of Philosophy; Emerging Technologies; Employee; Equipment; Evaluation; Experimental Designs; Faculty; Feedback; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Grant; Image; Image Cytometry; Immune; Immunophenotyping; Institutes; Lasers; Leadership; Licensing; Maintenance; Malignant Neoplasms; Manuscripts; Microfluidics; Monitor; NCI-Designated Cancer Center; Patients; Phenotype; Population; Preparation; Protocols documentation; Publications; Research; Research Personnel; Resource Sharing; Science; Scientist; Services; Site; Solid Neoplasm; Sorting - Cell Movement; Speed; Stains; Surveys; Suspensions; Technology; Time; Training; Work; cost effectiveness; experience; experimental study; instrument; instrumentation; investigator training; meetings; member; programs; repaired; tumor

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource offers Cancer Center members multi-parametric evaluation and high- speed enrichment of phenotypically distinct cell populations. The Core provides full-service high-speed cell sorting performed by expert operators, utilizing two BD Aria instruments in biosafety enclosures. For analytical cytometry, investigators have a choice of either full-service analysis by Core staff or receiving training and then independently using one of six analytical flow cytometers. Those analytical cytometers, available 24/7 to trained users, include two BD Fortessas, the newer of which is a 5-laser, 18-color instrument, and an ImageStreamX MkII imaging flow cytometer with four lasers and 12 imaging channels. Many of the Core's cytometers have autoloaders, enabling larger-scale experiments. Analysis software is available in the Core or via the Institutional site license. This broadly used Core is managed by the CC and receives support from the Institute for instrumentation purchase and maintenance. In the last funding period, three major technology platforms were acquired, and imaging flow cytometry was launched as a new service. The Core Director, Mr. Altman, and staff have combined experience of more than 30 years in NCI-designated Cancer Center Flow Cytometry Cores, and they provide comprehensive technical guidance at any stage of a project, from experimental design through manuscript preparation. The Flow Cytometry Core is broadly used, as in the last 5 years, it was used by 46 Cancer Center labs representing all three programs, and the Core contributed to at least 75 cancer-related publications.

摘要 – 流式细胞术共享资源 流式细胞术共享资源为癌症中心成员提供多参数评估和高 快速富集表型不同的细胞群。Core 提供全方位服务的高速细胞。 由专家操作员使用生物安全外壳中的两台 BD Aria 仪器进行分选。 细胞计数，研究人员可以选择由核心人员提供全方位服务分析或接受培训，然后 独立使用六台分析流式细胞仪中的一台 这些分析式细胞仪可全天候 (24/7) 接受培训。 用户，包括两台 BD Fortessas，其中较新的是一台 5 激光、18 色仪器和一台 ImageStreamX MkII 成像流式细胞仪具有四个激光器和 12 个成像通道 许多 Core 的细胞仪都具有。 自动加载器，可以在核心中或通过机构使用分析软件。 这个广泛使用的核心由 CC 管理并获得研究所的支持。 仪器仪表采购和维护在上一个资助期间，三个主要技术平台。 收购，并推出成像流式细胞术作为一项新服务 核心总监 Altman 先生和员工。 在 NCI 指定癌症中心流式细胞术核心领域拥有超过 30 年的综合经验，并且 他们在项目的任何阶段提供全面的技术指导，从实验设计到 手稿准备。流式细胞术核心得到广泛使用，在过去 5 年中，有 46 家使用它。 代表所有三个项目的癌症中心实验室，核心项目为至少 75 个与癌症相关的项目做出了贡献 出版物。