Flow Cytometry

流式细胞术

• 批准号: 10400710
• 负责人: Aniruddha J. Deshpande
• 金额: $ 21.23万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10400710
• 关键词: Antibodies; Cancer Center; Cancer Center Support Grant; Cell Separation; Cells; Color; Communication; Computer software; Contract Services; Core Facility; Cytometry; Data Analyses; Development; Doctor of Philosophy; Emerging Technologies; Employee; Equipment; Evaluation; Experimental Designs; Faculty; Feedback; Flow Cytometry; Flow Cytometry Shared Resource; Funding; Grant; Image; Image Cytometry; Immune; Immunophenotyping; Institutes; Lasers; Leadership; Licensing; Maintenance; Malignant Neoplasms; Manuscripts; Microfluidics; Monitor; NCI-Designated Cancer Center; Patients; Phenotype; Population; Preparation; Protocols documentation; Publications; Research; Research Personnel; Resource Sharing; Science; Scientist; Services; Site; Solid Neoplasm; Sorting - Cell Movement; Speed; Stains; Surveys; Suspensions; Technology; Time; Training; Work; cost effectiveness; experience; experimental study; instrument; instrumentation; investigator training; meetings; member; programs; repaired; tumor

ABSTRACT – FLOW CYTOMETRY SHARED RESOURCE The Flow Cytometry Shared Resource offers Cancer Center members multi-parametric evaluation and high- speed enrichment of phenotypically distinct cell populations. The Core provides full-service high-speed cell sorting performed by expert operators, utilizing two BD Aria instruments in biosafety enclosures. For analytical cytometry, investigators have a choice of either full-service analysis by Core staff or receiving training and then independently using one of six analytical flow cytometers. Those analytical cytometers, available 24/7 to trained users, include two BD Fortessas, the newer of which is a 5-laser, 18-color instrument, and an ImageStreamX MkII imaging flow cytometer with four lasers and 12 imaging channels. Many of the Core's cytometers have autoloaders, enabling larger-scale experiments. Analysis software is available in the Core or via the Institutional site license. This broadly used Core is managed by the CC and receives support from the Institute for instrumentation purchase and maintenance. In the last funding period, three major technology platforms were acquired, and imaging flow cytometry was launched as a new service. The Core Director, Mr. Altman, and staff have combined experience of more than 30 years in NCI-designated Cancer Center Flow Cytometry Cores, and they provide comprehensive technical guidance at any stage of a project, from experimental design through manuscript preparation. The Flow Cytometry Core is broadly used, as in the last 5 years, it was used by 46 Cancer Center labs representing all three programs, and the Core contributed to at least 75 cancer-related publications.

摘要 - 流式细胞仪共享资源 流式细胞仪共享资源提供了癌症中心成员多参数评估和高级评估 表型不同的细胞群体的速度富集。核心提供全方位服务的高速单元 由专家运营商执行的分类，使用两种BD ARIA仪器在生物安全围栏中进行分类。用于分析 细胞仪，调查人员可以选择由核心人员进行全方位服务分析，或者接受培训，然后选择 独立使用六个分析流式细胞仪之一。那些分析细胞仪，可用于24/7的训练 用户，包括两个BD Fortessas，其中的较新是5射线器，18色乐器和一个ImagestreamX MKII成像流式细胞仪，具有四个激光和12个成像通道。许多核心的细胞仪都有 自动加载器，实现大规模实验。分析软件可在核心或通过机构提供 现场许可证。该广泛使用的核心由CC管理，并得到研究所的支持 仪器购买和维护。在最后一个资金期间，三个主要的技术平台是 获得的，成像流式细胞仪是作为新服务启动的。核心主任，阿尔特曼先生和员工 在NCI指定的癌症中心流式细胞仪核心和 他们在项目的任何阶段提供全面的技术指导，从实验设计到 手稿准备。流式细胞仪核被广泛使用，因为在过去的5年中，它被46使用 代表这三个程序的癌症中心实验室，核心至少有75个与癌症有关 出版物。