Functional Genomics

功能基因组学

• 批准号: 10174821
• 负责人: Aniruddha J. Deshpande
• 金额: $ 30.6万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10174821
• 关键词: Adenoviruses; Adopted; Automation; Bioinformatics Shared Resource; Biological Assay; CRISPR library; CRISPR screen; CRISPR/Cas technology; Cancer Center; Cancer Center Support Grant; Cell Line; Cells; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communication; Computer software; Contract Services; Core Facility; Cultured Cells; Custom; Data; Data Analyses; Doctor of Philosophy; Employee; Engineering; Equipment; Experimental Designs; Faculty; Feedback; Gene Activation; Gene Family; Genetic; Genetic Screening; Genomics Shared Resource; Goals; Grant; Guide RNA; Image; Label; Laboratories; Leadership; Libraries; Maintenance; Malignant Neoplasms; Methods; MicroRNAs; Microscopy; Miniaturization; Mutation; Patients; Preparation; Publications; RNA Interference; Reagent; Research; Research Personnel; Resource Sharing; Science; Scientist; Services; Small Interfering RNA; Statistical Data Interpretation; Supporting Cell; Surveys; System; Technology; Time; Viral; Viral Vector; Work; assay development; base; cost effectiveness; design; exome; functional genomics; gene repression; genome-wide; high throughput screening; inhibitor/antagonist; meetings; member; nucleic acid delivery; programs; repaired; repository; screening; small hairpin RNA; small molecule libraries; tool; vector; whole genome

ABSTRACT – FUNCTIONAL GENOMICS SHARED RESOURCE The central goal of the Functional Genomics Shared Resource, which is managed by the Cancer Center, is to provide services and tools for Center members to perform RNAi and CRISPR-CAS9 genetic screens in cultured cells. Core staff interact with Center faculty members throughout their projects, starting with project conceptualization and feasibility assessment through to the final verification of targets identified in the screens. The Core utilizes the extensive amount of HTS equipment available in the Prebys Center and the Chemical Library Screening Core. In addition to the wealth of expertise brought by the staff, the Core serves as a repository for reagents, assays, and technical information, which are shared with Cancer Center researchers. The Core provides a variety of libraries for functional genomics analysis, including a genome-wide siRNA library, and comprehensive miRNA activator and inhibitor libraries, broad shRNA libraries, as well as targeted arrayed and genome-wide pooled CRISPR libraries. The Core's primary effort is dedicated to genetic screening via RNAi and CRISPR-Cas9, although significant time is also currently dedicated to engineering of cell lines. Within the Core, the main laboratory focuses on cellular genetics, while the viral vector laboratory specializes in high-quality vector preparation in a centralized facility with expert staff, and produces an array of sophisticated viral systems to enable nucleic acid delivery. Both laboratories are also actively involved in developing tools tailored to the specific needs of Center researchers. Functional Genomics operates in close connection with other Shared Resources at SBP. Thus, the Core utilizes automation and high-throughput imaging support from the adjacent Chemical Library Screening Shared Resource; interacts with the Bioinformatics Shared Resource to perform statistical analysis of the raw data and design custom CRISPR libraries; and uses the sequencing services of the Genomics Shared Resource to execute CRISPR-Cas9 screens. Overall, the Functional Genomics Core has strived to maintain its place at the forefront of cellular genetics and nucleic acid delivery by adopting state-of- the-art technologies, implementing methods to fill technology gaps, and adapting methods to fulfill the specific needs of the Cancer Center. In the past 5 years, the Core was used by a total of 45 Center members representing all three programs, and it supported at least 29 cancer-related publications by Cancer Center members.

摘要——功能基因组学共享资源 由癌症中心管理的功能基因组学共享资源的中心目标是 为中心成员提供在培养物中进行 RNAi 和 CRISPR-CAS9 遗传筛选的服务和工具 从项目开始，核心人员在整个项目中与中心教员进行互动。 概念化和可行性评估，直至屏幕中确定的目标的最终验证。 核心利用 Prebys 中心和化学中心提供的大量高温超导设备 图书馆筛选核心除了工作人员带来的丰富专业知识外，还充当存储库。 与癌症中心研究人员共享的试剂、测定和技术信息。 提供各种用于功能基因组学分析的文库，包括全基因组 siRNA 文库，以及 全面的 miRNA 激活剂和抑制剂库、广泛的 shRNA 库以及靶向阵列和 Core 的主要工作是通过 RNAi 和 CRISPR 进行基因筛选。 CRISPR-Cas9，尽管目前也投入了大量时间在核心内进行工程改造。 主实验室专注于细胞遗传学，病毒载体实验室专注于高质量载体 在由专家人员组成的集中设施中进行制备，并生产一系列复杂的病毒系统 两个实验室还积极参与开发适合核酸输送的工具。 中心研究人员的具体需求与其他共享基因组学密切相关。 SBP 的资源因此，核心利用了邻近的自动化和高通量成像支持。 化学库筛选共享资源；与生物信息学共享资源交互执行 对原始数据进行统计分析并设计定制CRISPR文库；并使用测序服务 执行 CRISPR-Cas9 筛选的基因组学共享资源 总体而言，功能基因组学核心具有。 通过采用最新状态，努力保持其在细胞遗传学和核酸递送领域的前沿地位 最先进的技术、填补技术空白的实施方法以及适应特定方法的方法 在过去 5 年中，共有 45 名代表癌症中心的成员使用了核心。 它支持所有三个项目，并支持癌症中心成员发表的至少 29 篇与癌症相关的出版物。