Crosstalk of immune and nervous systems by 3-D imaging

3D 成像对免疫系统和神经系统的串扰

• 批准号: 10044312
• 负责人: NAKANISHI Mamoru
• 金额: $ 3.39万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10044312/
• 关键词: Allergy; Mast cells; Bradykinin; Confocal laser scanning microscopy; Calcium ion; NK-1 antagonist; Substance P; Nerves; サブスタンスP; 好塩基球; 共焦点レーザ顕微鏡; アンタゴニスト

Communication between nerves and mast cells is a prototypic demonstration of neuroimmune interaction. However, whether mast cell activation occurs as a direct response to neuronal activation or requires an intermediary cell is unclear. Addressing this issue, we used an in vitro coculture approach comprising cultured murine superior cervical ganglia and rat leukemia basophilic cells (RBLs ; possesses properties of mucosal-type mast cells). Following loading with the calcium fluorophore, Fluo-3, neurite-RBL units (separated by <50nm) were examined by confocal laser scanning microscopy. Addition of bradykinin, or scorpion venom, dose-dependently elicited neurite activation (i.e., CaィイD12+ィエD1 mobilization) and, after a lag period, RBL CaィイD12+ィエD1 mobilization. Neither bradykinin nor scorpion venom had any direct effect on the RBLs in the absence of neurites. Addition of a neutralizing substance P Ab or a neurokinin (NK)-1 receptor antagonist, but not an NK-2 receptor antagonist, dose-dependently prevented the RBL activation that resulted as a consequence of neural activation by either bradykinin or scorpion venom. These data illustrate that nerve-mast cell cross-talk can occur in the absence of an intermediary transducing cell and that the neuropeptide substance P, operating via NK-1 receptors, is an important mediator of this communication. Our findings have implications for the neuroimmune signaling cascades that are likely to occur during airways inflammation, intestinal hypersensitivity, and other conditions in which mast cells feature.

神经与肥大细胞之间的通信是神经免疫相互作用的原型示范。通过共聚焦激光扫描显微镜检查Bradykinin或Scorpion Venom，剂量依赖性的活化，RBL Ca I D12+YIE D1均未对radykinin nobilization进行任何直接效果。 PAR AB或Aneurokinin（NK）-1受体拮抗剂ECEPTOR拮抗剂，剂量急剧防止RBL激活作为Bradykinin或Scorpion毒液的神经激活的结构，这些数据表明，这些数据表明了可能发生的神经乳细胞交叉词可能会发生。通过NK-1受体运行的IRAIR转导细胞和神经肽物质P是这种通信的进口介体。

项目成果

R.Nakamura: "Atomic force microscopy for the cellular localization of secretory granules and coated pits in rat basophilic leukemia cells after antigen stimulation." Bioimages. 6. 69-75 (1998)

R.Nakamura：“原子力显微镜用于抗原刺激后大鼠嗜碱性白血病细胞中分泌颗粒和包被凹坑的细胞定位。”

Suzuki, R. et al.: "Direct neurite-mast cell (RBL) communication in vitro occurs via the neuropeptide substance P"J. Immunol.. 163. 2410-2415 (1999)

Suzuki, R. 等人：“体外直接神经突-肥大细胞 (RBL) 通讯是通过神经肽物质 P 发生的”J.

R. Nakamura: "Atomic force microscopy to study the degranulation in rat peritoneal mast cell activation."Immunol. Lett.. 69. 307-310 (1999)

R. Nakamura：“原子力显微镜研究大鼠腹膜肥大细胞激活中的脱颗粒。”免疫。

R. Suzuki: "Direct neurite-mast cell (RBL) communication in vitro occurs via the neuropeptide substance P."J. Immunol.. 163. 2410-2415 (1999)

R. Suzuki：“体外直接神经突-肥大细胞 (RBL) 通讯是通过神经肽物质 P 发生的。”J.

中西 守 他: "バイオイメージングの最先端"先端医療技術研究所. 324 (1999)

Mamoru Nakanishi 等人：“生物成像的前沿”先进医疗技术研究所 324 (1999)。