Immune-neuro Cross-talk with Confocal Laser Scanning Microscopy

共焦激光扫描显微镜的免疫神经串扰

• 批准号: 15390017
• 负责人: NAKANISHI Mamoru
• 金额: $ 9.79万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15390017/
• 关键词: Mast cell; SCG; Laser microscopy; Substance P; N-cadherin; SynCAM; ATP; RBL; 神経初代培養細胞; 細胞間相互作用; ノルアドレナリン; カドヘリン; 接着分子; 免疫細胞; 神経細胞; クロストーク; カルシウムイオン

Communication between nerves and mast cells is a prototypic demonstration of neuro-immune interaction. Previously, we showed clearly that nerve-mast cell cross-talk can occur bidirectionally in the absence of an intermediary transducing cell using an in vitro co-culture approach and calcium imaging. In terms of bidirectional communication, we demonstrated that the neuropeptide substance P is an important mediator in efferent communication (i.e. neuron-to-mast cell), but the mediators in afferent communication (i.e. mast cell-to-neuron) have been still unknown. Then, we have studied the mediators to activate neurites (superior cervical ganglia ; SCG) cocultured with mast cells (rat basophilic leukemia cells ; RBLs). Addition of antigen to the cocultures of SCG and RBLs elicited the increase in the [Ca^<2+>]_i in RBLs and after a lag period induced the increase in the [Ca^<2+>]_i in SCG neuretes extending at a long distance (>150μm) from the site where the RBLs attached to the neurites. The pretreatment of pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS) or apyrase, which is a purinergic receptor 2 antagonist or an ATP hydrolyzing enzyme, respectively, prevented the calcium signals in SCG neuritis which were resulted as a consequence of RBL activation, but the pretreatment of chlorophenilamine or ketanserin, which is a histamine or serotonin receptor antagonist, respectively, did not. These results indicated that the ATP released from activated mast cells functioned as an important mediator in afferent communication between neurites and mast cells, but not histamine and serotonin. In addition, we found the roles of SynCAM and N-cadherin in the synaps-like structures between mast cells and neuritis.

神经和肥大细胞之间的通信是神经免疫相互作用的原型示范。 efentication的重要介体。e。与肥大细胞EMIA细胞共培养； 2+>] _在学校中附着的RBL。 ，但是这些ATP释放的肥大细胞释放的氯酚或酮素的预处理并没有在传统的介体中起作用。肥大细胞之间的突触状结构。

项目成果

Noguchi, S., Hirashima, N., Furuno, T., Nakanishi, M.: "Remarkable induction of apoptosis in cancer cells by a novel cationic liposome comlexed with a bcl-2 antisense oligonucleotide"J Control Release. 7,88. 313-320 (2003)

Noguchi, S.、Hirashima, N.、Furuno, T.、Nakanishi, M.：“与 bcl-2 反义寡核苷酸复合的新型阳离子脂质体显着诱导癌细胞凋亡”J Control Release。

Flotinin-1 regulates IgE receptor-mediated signaling in RBL-2H3 cells

Flotinin-1 调节 RBL-2H3 细胞中 IgE 受体介导的信号传导

• 发表时间: 2006
• 期刊: J.Immunol. (印刷中)
• 作者: Kato;N.;Nakanishi;M.;Hirashima;N.
• 通讯作者: N.

Interaction between nerves and mast cells is mediated by the SgIGSF/SynCAM adhesion molecule.

神经和肥大细胞之间的相互作用是由 SgIGSF/SynCAM 粘附分子介导的。

• 发表时间: 2005
• 期刊: J.Immunol. (in press)
• 作者: Furuno;T.;Ito;A.;Koma;Y.;Watabe;K.;Yokozaki;H.;Bienenstock;J.;Nakanishi;M.;Kitamura;Y.
• 通讯作者: Y.

Remarkable induction of apoptosis in cancer cells by a novel cationic liposome complexed with a bcl-2 antisense oligonucleotide.

• DOI: 10.1016/s0168-3659(02)00484-4
• 发表时间: 2003-03
• 期刊: Journal of controlled release : official journal of the Controlled Release Society
• 作者: Shoichiro Noguchi;N. Hirashima;T. Furuno;M. Nakanishi

Phosphatidylinositol 4-kinase type IIα is responsible for degranulation in mast cells

IIα 型磷脂酰肌醇 4 激酶负责肥大细胞的脱颗粒

• 发表时间: 2006
• 期刊: Inflammation Res., (in press)
• 作者: Ishihara;Y.;Furuno;T.;Nakanishi;M.
• 通讯作者: M.