Bioimaging and theory for drug absorption in living cells

活细胞药物吸收的生物成像和理论

基本信息

批准号：
06304044
负责人：
NAKANISHI Mamoru
金额：
$ 2.37万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06304044/
关键词：
Durg absorption Confocal Microscopy Scanning Probe Microscopy Investigation of New Drug Absorption and Transportion Optical microscopic technique 顕微光学技術経皮吸収初回通回効果高分子薬物

项目摘要

A renaissance and a revolution in light microscopy are now under way. Several new technologies, used in conjunction with the light microscope, have yeilded a new way of seeing and measuring drugs in living cells. One major contribution to the new methodology is the use of computers for the digital processing and analysis of images. The second vital innovation in the new microscopy is the development of multicolor fluorescent dye molecules which represent a modern version ofselective stains relies on by earlier generations of microscopists. In this projects we have tried to study bioimaging for drug a bsorption in living cells by new light microscopes. We obtained various new findings for this subject. They were the studies of the surface expression of CD63 antigen in P815 mastocytoma cells by transfected IgE receptors, the conducance change in phospholipid bilayr membrace by an electroneutral ionophore (monensin), the application and prospects for physiologically based pharmacokinetic models involving pharmaceutical agents, the galactosylated proteins recognized by the liver according to the surface density of galactose, and cloning and characterization of rat H^+/peptidecotransporter mediating absorption of beta-lactam antibiotics in the intestine and kidney. Thus, the present Grant-in-Aid for Co-operative Research was able to obtain an extreemly high gool in the field of drug absorption in living cells.

光学显微镜的复兴和革命正在进行中。几种新技术与光学显微镜结合使用，产生了一种观察和测量活细胞中药物的新方法。新方法的一个主要贡献是使用计算机对图像进行数字处理和分析。新型显微镜的第二个重要创新是多色荧光染料分子的开发，它代表了前几代显微镜学家所依赖的现代版本的选择性染色。在这个项目中，我们尝试通过新型光学显微镜研究活细胞中药物吸收的生物成像。我们获得了关于这个主题的各种新发现。主要研究转染IgE受体在P815肥大细胞瘤细胞表面表达CD63抗原、电中性离子载体（莫能菌素）对磷脂双层膜的电导变化、基于生理学的药代动力学模型的应用和前景，涉及药剂、半乳糖基化根据半乳糖表面密度被肝脏识别的蛋白质，以及大鼠的克隆和表征H^+/肽转运蛋白介导肠道和肾脏中 β-内酰胺抗生素的吸收。因此，本次合作研究资助项目能够在活细胞药物吸收领域获得极高的成果。