Crosstalk of immune and nervous systems by 3-D imaging

3D 成像对免疫系统和神经系统的串扰

基本信息

批准号：
08044312
负责人：
NAKANISHI Mamoru
金额：
$ 4.74万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044312/
关键词：
Nervous systems Immune systems Neurotransmitters Mast cells RBL-2H3 Bradykinin Scorpion venom Substance P 共焦点レーザ顕微鏡神経細胞神経節好塩基球神経突起 NGF

项目摘要

It has been well known for a long time that the mind may influence the body. Related to this, the idea of bidirectional communication between nervous and immune systems has been discussed extensively. It is based on experimental evidence showing that neurotransmitters and neuronal activity can affect immune cells and induce immunological responses. On the other hand, immune activation can influence the function of the central and autonomic nervous systems. Here, to understand the molecular mechanism of these kinds of communication between the immune and nervous systems we established a time culture model of murine symphathetic neurons co-cultured with rat basophilic leukaemia (RBL-2H3) cells (homologues of mucosal mast cells) in collaboration with Prof.John Bienenstock at McMaster University. In co-cultures of up 3 days, light microscopy identified neurite contacts with RBL-2H3 cells. Then, we have studied the calcium signals in both nerve and mast cells after the interaction by confocal laser scanning microscopy. Fluo-3 fluorescence intensity in the neurites increased after stimulation with bradykinin. this indicated that calcium ion concentration increased in the neurites with bradykinin stimulation. After that calcium ion concentration increased also in RBL-2H3 cells which were contacted with neurites. However, such kinds of calcium signals were never observed in RBL-2H3 cells which were not contacted with the neurites. Similar kinds of calcium signals were also induced in RBL-2H3 cells when the neurites were stimulated with scorpion venom. By the experiments using anti-substance P antibody, we found that substance P,which were released from the neurites, triggered these calcium signals in RBL-2H3 cells.

长期以来，人们都知道思想可以影响身体。与此相关，神经系统和免疫系统之间双向通信的想法已被广泛讨论。它基于实验证据，表明神经递质和神经元活动可以影响免疫细胞并诱导免疫反应。另一方面，免疫激活可以影响中枢和自主神经系统的功能。在这里，为了了解免疫系统和神经系统之间此类通讯的分子机制，我们建立了小鼠交感神经元与大鼠嗜碱性白血病 (RBL-2H3) 细胞（粘膜肥大细胞的同源物）共培养的时间培养模型与麦克马斯特大学的 John Bienenstock 教授合作。在长达 3 天的共培养中，光学显微镜识别出神经突与 RBL-2H3 细胞的接触。然后，我们通过共焦激光扫描显微镜研究了神经细胞和肥大细胞相互作用后的钙信号。用缓激肽刺激后，神经突中的 Fluo-3 荧光强度增加。这表明在缓激肽刺激下，神经突中的钙离子浓度增加。此后，与神经突接触的RBL-2H3细胞中的钙离子浓度也增加。然而，在不与神经突接触的RBL-2H3细胞中从未观察到此类钙信号。当用蝎毒刺激神经突时，RBL-2H3 细胞中也会诱导类似的钙信号。通过使用抗P物质抗体的实验，我们发现从神经突释放的P物质触发了RBL-2H3细胞中的这些钙信号。