Crosstalk of immune and nervous systems by 3-D imaging

3D 成像对免疫系统和神经系统的串扰

• 批准号: 08044312
• 负责人: NAKANISHI Mamoru
• 金额: $ 4.74万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044312/
• 关键词: Nervous systems; Immune systems; Neurotransmitters; Mast cells; RBL-2H3; Bradykinin; Scorpion venom; Substance P; 共焦点レーザ顕微鏡; 神経細胞; 神経節; 好塩基球; 神経突起; NGF; Nervous systems; Immune systems; Neurotransmitters; Mast cells; RBL-2H3; Bradykinin; Scorpion venom; Substance P; 共焦点レーザ顕微鏡; 神経細胞; 神経節; 好塩基球; 神経突起; NGF

It has been well known for a long time that the mind may influence the body. Related to this, the idea of bidirectional communication between nervous and immune systems has been discussed extensively. It is based on experimental evidence showing that neurotransmitters and neuronal activity can affect immune cells and induce immunological responses. On the other hand, immune activation can influence the function of the central and autonomic nervous systems. Here, to understand the molecular mechanism of these kinds of communication between the immune and nervous systems we established a time culture model of murine symphathetic neurons co-cultured with rat basophilic leukaemia (RBL-2H3) cells (homologues of mucosal mast cells) in collaboration with Prof.John Bienenstock at McMaster University. In co-cultures of up 3 days, light microscopy identified neurite contacts with RBL-2H3 cells. Then, we have studied the calcium signals in both nerve and mast cells after the interaction by confocal laser scanning microscopy. Fluo-3 fluorescence intensity in the neurites increased after stimulation with bradykinin. this indicated that calcium ion concentration increased in the neurites with bradykinin stimulation. After that calcium ion concentration increased also in RBL-2H3 cells which were contacted with neurites. However, such kinds of calcium signals were never observed in RBL-2H3 cells which were not contacted with the neurites. Similar kinds of calcium signals were also induced in RBL-2H3 cells when the neurites were stimulated with scorpion venom. By the experiments using anti-substance P antibody, we found that substance P,which were released from the neurites, triggered these calcium signals in RBL-2H3 cells.

很长一段时间以来，心灵可能会影响身体。与此相关的是，已经广泛讨论了神经和免疫系统之间双向交流的想法。它基于实验证据表明，神经递质和神经元活性会影响免疫细胞并诱导免疫学反应。另一方面，免疫激活会影响中心神经系统和自主神经系统的功能。在这里，为了了解免疫系统和神经系统之间这些这种交流的分子机制，我们建立了与大鼠嗜碱性白血病（RBL-2H3）细胞（粘膜同源物的同源物）（粘膜同源物）（粘膜同源物）的时间培养模型与McMcMmaster University的Johnbienstock教授合作。在增加3天的共培养中，光学显微镜鉴定出与RBL-2H3细胞的神经突接触。然后，我们通过共聚焦激光扫描显微镜研究了神经和肥大细胞中的钙信号。在用心动激肽刺激后神经突的荧光强度增加。这表明钙离子浓度在心动激肽刺激的神经突中升高。之后，在与神经突接触的RBL-2H3细胞中钙离子浓度也增加。但是，在RBL-2H3细胞中从未观察到这种钙信号，这些信号未与神经突接触。当用蝎子毒液刺激神经突在RBL-2H3细胞中还诱导了类似的钙信号。通过使用抗固定P抗体的实验，我们发现从神经突释放的物质P触发了RBL-2H3细胞中的这些钙信号。