Excellent biocompatibility of MPC polymers as biomaterials evaluated by fibroblast and HL-60 cells.

通过成纤维细胞和 HL-60 细胞评估 MPC 聚合物作为生物材料具有优异的生物相容性。

• 批准号: 09680847
• 负责人: WATANABE Akihiko
• 金额: $ 1.86万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09680847/
• 关键词: MPC Copolymer; Biocompatibility; Cell culture; Protein adsorption; Ne ion injection; Cytokine product; Neイオン照射; 医用ポリマー; MPCポリマー; 傾斜細胞応答性材料; 生体適合性材料; 高機能性材料の合成; イオン注入材料表面; 電子顕微鏡

Attachment of cells to a biomaterial surface are initiated the protein adsorbed there. When protein adsorption are prevented, cellular attachment must be minimized. Copolymers of methacryloyloxyethyl phosphorylcholine (MPC) have affinity for phospholipids due to the phosphorylcholine polar groups on surface. It has been found that they are useful biomaterials for blood contacting devices as they do not induce platelet activation and blood coagulation.We realized that MPC surfaces inhibited attachment oh HL-60 cells and moreover they did not promote cytokine production. This result suggested that the MPC surface did not stimulate the cells. On the other hand conventional biomaterials promote accumulation of cytokine in the cells. We could conclude that MPC is very excellent biomaterials which do not give adverse effect on the cells. Further, this evaluation method must be applicable to examine biocompatibility of biomaterials. Another interesting result was fibroblast cell could not attack on the surface coated with MPC copolymers. On the other hand, when the coating was removed by Ne ion injection, they could attach and prolify there. Protein adsorption here was confirmed also.

细胞与生物材料表面的附着是由那里吸附的蛋白质引发的。当阻止蛋白质吸附时，必须尽量减少细胞附着。甲基丙烯酰氧基乙基磷酰胆碱（MPC）的共聚物由于表面上的磷酰胆碱极性基团而对磷脂具有亲和力。已发现它们是用于血液接触装置的有用生物材料，因为它们不会诱导血小板活化和血液凝固。我们意识到MPC表面抑制HL-60细胞的附着，而且它们不促进细胞因子的产生。这一结果表明 MPC 表面不会刺激细胞。另一方面，传统的生物材料促进细胞内细胞因子的积累。我们可以得出结论，MPC是非常优秀的生物材料，不会对细胞产生不利影响。此外，该评估方法必须适用于检查生物材料的生物相容性。另一个有趣的结果是成纤维细胞不能攻击涂有 MPC 共聚物的表面。另一方面，当通过氖离子注射去除涂层时，它们可以在那里附着并增殖。此处的蛋白质吸附也得到证实。

项目成果

鈴木嘉昭: "細胞外マトリックスへのイオンビーム照射と医療応用" アイオニクス. 24・7. 3-11 (1998)

铃木义明：“细胞外基质的离子束照射和医学应用”Ionics 24・7（1998）。

Yasuhiko Iwasaki: "The effect of the chemical of the phospholipid polymer on fibronectin adsorption and fibroblast adhesion on the gradient phospholipid surface"Biomaterials. 20. 2185-2191 (1999)

Yasuhiko Iwasaki：“磷脂聚合物的化学成分对梯度磷脂表面上的纤连蛋白吸附和成纤维细胞粘附的影响”生物材料。

渡辺昭彦、他: "MPC共重合体上での培養細胞の挙動の制御"東京医科歯科大学・生体材料工学研究所報告. 33. 34-39 (1999)

Akihiko Watanabe 等人：“控制 MPC 共聚物上培养细胞的行为”，东京医科齿科大学生物材料工程研究所，报告 33. 34-39 (1999)。

Yasuhiko Iwasaki: "The effect of the chemical structure of the phospholipid polymer on fibronectin adsorption and fibroblast adhesion on the gradient phospholipid surface"Biomaterials. 20. 2185-2191 (1999)

Yasuhiko Iwasaki：“磷脂聚合物的化学结构对梯度磷脂表面上纤连蛋白吸附和成纤维细胞粘附的影响”生物材料。

渡辺昭彦: "MPC共重合体上での繊維芽細胞の接着及び増殖の制御"IONICS -イオンの科学と技術-. 24. 13-19 (1998)

Akihiko Watanabe：“MPC 共聚物上成纤维细胞粘附和增殖的控制”IONICS - 离子科学与技术 - 24. 13-19 (1998)。