Functional Characteristics of a Novel Transporter Specifically Expressed in the Endoplasmic Reticulum in Dendritic Cells and its Role in Immune System

树突状细胞内质网特异性表达的新型转运蛋白的功能特性及其在免疫系统中的作用

• 批准号: 23659085
• 负责人: YUASA Hiroaki
• 金额: $ 2.41万
• 依托单位: Nagoya City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23659085/
• 关键词: トランスポーター; 樹状細胞; 小胞体; 免疫; 抗アレルギー薬; 創薬標的

Functional characteristics of human vesicular organic anion transporter 1 (VOAT1) were examined by assessing the cellular uptake (accumulation in the endoplasmic reticulum) of 5-aminofluorescein (5-AF) as a fluorescent probe substrate, based on the observation of fluorescent microscopic images. 5-AF uptake was found to be specifically inhibited by several anti-allergic drugs, anti-inflammatory drugs, and endogenous fatty acids, including their oxidative products. These findings suggest that VOAT1 might be involved in the transport of lipid mediators and, thereby, play a role in inflammatoric and immunologic processes. It might also be involved in the pharmacologic action of anti-inflammatory drugs. Thus, we could obtain valuable information about the functional characteristics of VOAT1, although it needs to be further validated by quantitative assessments.

通过观察荧光显微图像，评估作为荧光探针底物的 5-氨基荧光素 (5-AF) 的细胞摄取（在内质网中的积累），从而检查人囊泡有机阴离子转运蛋白 1 (VOAT1) 的功能特征。研究发现，多种抗过敏药物、抗炎药物和内源性脂肪酸（包括它们的氧化产物）会特异性抑制 5-AF 的摄取。这些发现表明 VOAT1 可能参与脂质介质的转运，从而在炎症和免疫过程中发挥作用。它还可能参与抗炎药物的药理作用。因此，我们可以获得有关 VOAT1 功能特征的有价值的信息，尽管它需要通过定量评估进一步验证。