Regulation of inflammation by stress proteins

应激蛋白调节炎症

• 批准号: 24790077
• 负责人: TANAKA Kenichiro
• 金额: $ 2.83万
• 依托单位: Keio University (2013)Kumamoto University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790077/
• 关键词: ストレスタンパク質

In this study, we have examined the role of HSP70 in gastric ulcer healing. Gastric ulcers were produced by focal and serosal application of acetic acid. Expression of HSP70 was induced in both the gastric ulcer margin and granulation tissue. Compared with wild-type mice, gastric ulcer healing was accelerated in transgenic mice expressing HSP70, and both cell proliferation at the gastric ulcer margin and angiogenesis in granulation tissue were enhanced. Oral administration of geranylgeranylacetone, an inducer of HSPs, to wild-type mice, either prior to or after ulcer formation, not only induced expression of HSP70 in the stomach but also accelerated gastric ulcer healing. On the other hand, oral administration of purified recombinant HSP70 prior to the ulcer formation, but not after formation, stimulated gastric ulcer healing. This study provides the first evidence that HSP70 accelerates gastric ulcer healing.

在这项研究中，我们研究了 HSP70 在胃溃疡愈合中的作用。胃溃疡是通过局部和浆膜应用乙酸产生的。在胃溃疡边缘和肉芽组织中均诱导了 HSP70 的表达。与野生型小鼠相比，表达HSP70的转基因小鼠的胃溃疡愈合加速，胃溃疡边缘的细胞增殖和肉芽组织的血管生成均增强。在溃疡形成之前或之后，给野生型小鼠口服热休克蛋白诱导剂香叶基香叶基丙酮，不仅诱导胃中HSP70的表达，而且加速了胃溃疡的愈合。另一方面，在溃疡形成之前而非形成之后口服纯化的重组HSP70会刺激胃溃疡愈合。这项研究提供了 HSP70 加速胃溃疡愈合的第一个证据。

项目成果

Comparison of pharmacokinetics between loxoprofen and its derivative with lower ulcerogenic activity, fluoro-loxoprofen

洛索洛芬及其具有较低致溃疡活性的衍生物氟洛索洛芬的药代动力学比较

• 发表时间: 2013
• 期刊: Drug Metab. Pharmacokinet
• 作者: Yamakawa;N.;Suemasu;S.;Watanabe;H.;Tahara;K.;Tanaka;K.;Okamoto;Y.;Ohtsuka;M.;Maruyama;T. and Mizushima;T.
• 通讯作者: T.

Effects of Lecithinized Superoxide Dismutase and/or Pirfenidone Against Bleomycin-Induced Pulmonary Fibrosis

• DOI: 10.1378/chest.11-2879
• 发表时间: 2012-10-01
• 期刊: CHEST
• 影响因子: 9.6
• 作者: Tanaka, Ken-Ichiro;Azuma, Arata;Mizushima, Tohru
• 通讯作者: Mizushima, Tohru

Identification of a unique nsaid, fluoro-loxoprofen with gastroprotective activity.

• DOI: 10.1016/j.bcp.2012.09.016
• 发表时间: 2012-12
• 期刊: Biochemical pharmacology
• 影响因子: 5.8
• 作者: Shintaro Suemasu;N. Yamakawa;T. Ishihara;Teita Asano;Kayoko Tahara;Ken-ichiro Tanaka;H. Matsui;Yoshinari Okamoto;M. Otsuka;K. Takeuchi;Hidekazu Suzuki;T. Mizushima

Ameliorative Effect of Mepenzolate Bromide against Pulmonary Fibrosis

• DOI: 10.1124/jpet.114.213009
• 发表时间: 2014-07-01
• 期刊: JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
• 影响因子: 3.5
• 作者: Kurotsu, Shota;Tanaka, Ken-ichiro;Mizushima, Tohru
• 通讯作者: Mizushima, Tohru

Synthesis and biological comparison of enantiomers of mepenzolate bromide, a muscarinic receptor antagonist with bronchodilatory and anti-inflammatory activities

• DOI: 10.1016/j.bmc.2014.04.029
• 发表时间: 2014-07-01
• 期刊: BIOORGANIC & MEDICINAL CHEMISTRY
• 影响因子: 3.5
• 作者: Yamashita, Yasunobu;Tanaka, Ken-Ichiro;Mizushima, Tohru
• 通讯作者: Mizushima, Tohru