Bio-synthesis of poly(lactic acid) by wild type PHA-producing bacteria

野生型PHA产生菌生物合成聚乳酸

• 批准号: 23655145
• 负责人: AOKI Takashi
• 金额: $ 2.41万
• 依托单位: Kyoto Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655145/
• 关键词: バイオベースポリマー; 生体材料; 生分解性・生体吸収性; 応用微生物; バイオテクノロジー; 微生物合成

Poly(lactic acid) (PLA) is one of bio-based polymers synthesized from renewable resources in plants. We have recently found that PLA oligomers existed in a microbial cultured system of Ralstonia eutropha (R. eutropha), which is known to form other aliphatic polyester, poly(3-hydroxybutyrate) within its body. This research aimed to find optimal cell-culture conditions for both better mass production and higher molecular weight of PLA, establishing the suitable accumulation manner of the polyester under mild conditions. PLA obtained from R. eutropha culture system was found to composed of D-formed lactic acid repeating units, while that from other strain cell, Bacillus megaterium, contained D- and L-formed ones. The optical purity of lactic acid units in PLA depended on the cell strains. It was also found that our cell conditions could not result in large mass production or high molecular weight of PLA. Even now, we are trying to find out the optimal cell-culture conditions.

聚乳酸（PLA）是从植物中可再生资源合成的基于生物的聚合物之一。我们最近发现，在其体内形成其他脂肪族聚酯，聚（3-羟基丁酸酯）的微生物培养系统中存在PLA低聚物。这项研究旨在为更好的质量产生和更高的PLA分子量找到最佳的细胞培养条件，从而在轻度条件下建立了聚酯的合适积累方式。发现从富富洛氏菌培养系统获得的PLA由D形式的乳酸重复单元组成，而来自其他应变细胞的Megaterium含有D和L形成的单位。 PLA中乳酸单位的光纯度取决于细胞菌株。还发现我们的细胞条件不能导致PLA的质量大量产生或高分子量。即使是现在，我们仍在尝试找出最佳的细胞培养条件。

项目成果

PHB生産菌によるポリ乳酸の生合成挙動の解明

阐明 PHB 产生菌的聚乳酸生物合成行为

• 发表时间: 2011
• 作者: 吉岡良真;山中健志;李贊雨;青木隆史;木村良晴
• 通讯作者: 木村良晴

植物由来プラスチックであるポリ乳酸の微生物合成法の検討

植物基塑料聚乳酸的微生物合成方法研究

• 发表时间: 2011
• 作者: Y. Imura;N. Shimojuh;K. Moriyama;and H. Togo;M. Unno;吉岡良真・山中健志・青木隆史・木村良晴
• 通讯作者: 吉岡良真・山中健志・青木隆史・木村良晴