Development of DNA microarray for characterization of gene network systems of fish and shellfish

开发用于表征鱼类和贝类基因网络系统的 DNA 微阵列

• 批准号: 15108003
• 负责人: AOKI Takashi
• 金额: $ 67.23万
• 依托单位: Tokyo University of Marine Science and Technology (2004-2007)東京水産大学 (2003)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (S)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15108003/
• 关键词: Japanese flounder; kuruma shrimp; black tiger shrimp; red seabream; yellowtail; cDNA microarray; gene expression profiling; immune and biodefense; 耐病性

We conducted expressed sequence tag analysis of yellowtail Seriola quinqueradiata, Japanese flounder Paralichthys olivaceus, red seabream, Pagrus major, kuruma shrimp Marsupenaeus japonicus and black tiger shrimp Penaeus monodon. We were able to successfully develop complementary DNA (cDNA) microarray slides for yellowtail, Japanese flounder, red seabream, kuruma shrimp and black tiger shrimp consisting of 1,034, 1,965, 997, 754, and 1,282 unique cDNAs, respectively. These array slides were used and were proven to be effective in the analysis of gene expression during immunostimulation with lipopolysaccharides (LPS), concanavaline A (conA), poly IC, and peptidoglycan (PG). These slides were also used in the development and evaluation of vaccines designed against fish pathogens. In addition, For Japanese flounder, resistant strains showed 2-fold up-regulation of antigen presentation-related genes following Edwardsiella tarda infection as compared to sensitive strains. Overall, our study shows that transcriptomics such as microarray is an effective biomarker tool in studying host-pathogen relationships and possibly for drug development.

我们对黄尾鰤、牙鲆、牙鲆、红鲷、真鲷、日本库鲁玛虾、斑节对虾进行了表达序列标签分析。我们成功开发了黄尾鱼、比目鱼、红鲷、车虾和黑虎虾的互补 DNA (cDNA) 微阵列载玻片，分别包含 1,034、1,965、997、754 和 1,282 个独特的 cDNA。这些阵列载玻片被用于分析脂多糖 (LPS)、刀豆蛋白 A (conA)、聚 IC 和肽聚糖 (PG) 免疫刺激过程中的基因表达，并被证明是有效的。这些幻灯片还用于开发和评估针对鱼类病原体的疫苗。此外，对于日本比目鱼，与敏感菌株相比，在迟缓爱德华氏菌感染后，抗性菌株表现出抗原呈递相关基因的上调 2 倍。总体而言，我们的研究表明，微阵列等转录组学是研究宿主-病原体关系并可能用于药物开发的有效生物标志物工具。

项目成果

Molecular cloning, gene structure and expression of two CC chemokines from Japanese flounder Paralichthys olivaceus

• DOI: 10.1046/j.1444-2906.2003.00728.x
• 发表时间: 2003-10
• 期刊: Fisheries Science
• 影响因子: 1.9
• 作者: Rutch Khattiya;I. Hirono;T. Aoki

Differentially expressed genes in hemocytes of Vibrio harveyi-challenged shrimp Penaeus monodon.

• DOI: 10.5483/bmbrep.2006.39.1.026
• 发表时间: 2006-01
• 期刊: Journal of biochemistry and molecular biology
• 作者: Kunlaya Somboonwiwat;Premruethai Supungul;V. Rimphanitchayakit;T. Aoki;I. Hirono;A. Tassanakajon

Identification of a novel C-type lectin gene in Japanese flounder, Paralichthys olivaceus.

• DOI: 10.1016/j.fsi.2007.05.002
• 发表时间: 2007-11
• 期刊: Fish & shellfish immunology
• 影响因子: 4.7
• 作者: H. Kondo;Andrew Gung Yeu Tzeh;I. Hirono;T. Aoki

Bulk isolation of immune response-related genes by expressed sequenced tags of Japanese flounder Paralichthys olivaceus leucocytes stimulated with Con A/PMA

• DOI: 10.1006/fsim.2002.0448
• 发表时间: 2003-05-01
• 期刊: FISH & SHELLFISH IMMUNOLOGY
• 影响因子: 4.7
• 作者: Nam, BH;Hirono, I;Aoki, T
• 通讯作者: Aoki, T

Induction of antiviral state in fish cells by Japanese flounder, Paralichthys olivaceus, interferon regulatory factor-1.

• DOI: 10.1016/j.fsi.2004.12.008
• 发表时间: 2005-07
• 期刊: Fish & shellfish immunology
• 影响因子: 4.7
• 作者: C. Caipang;I. Hirono;T. Aoki