Vial impact on prokaryotic population dynamics in deep groundwater

对深层地下水中原核生物种群动态的重要影响

• 批准号: 23651011
• 负责人: KATO Kenji
• 金额: $ 2.5万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23651011/
• 关键词: 物質循環; ウイルス; ウィルス

Observations were carried out by using boreholes drilled in sedimentary geological setting in Horonobe, Hokkaido. Groundwater obtained from 140 m deep contained 4.5 ×103±1.7 ×103mL-1virus-like particles. Incubation experiment conducted pseudo in situ condition performed shortly after sampling showed that viral control of prokaryotic abundance was observed in 80 hours of incubation. A picture of virus-like particle was successfully taken by using Transparent Electron Microscopy, which showed the candidate of the virus-like particle was Caudovirales-like.

利用在北海道幌延市沉积地质环境中钻取的钻孔进行观测，该地下水含有 4.5 ×103±1.7 ×103mL-1 的病毒样颗粒，采样后不久进行的模拟原位条件下的孵化实验表明。在80小时的培养过程中观察到病毒对原核生物丰度的控制，使用透明电子成功拍摄了病毒样颗粒的照片。显微镜检查显示，候选病毒颗粒与有尾病毒目类似。