Impact of green house effect on marine bacteria with 5C

5C下温室效应对海洋细菌的影响

基本信息

批准号：
16310024
负责人：
KATO Kenji
金额：
$ 7.42万
依托单位：
Shizuoka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

An intensive study was carried out in Simizu-Port of Suruga Bay throughout 2004 and 2005. Together with the results of preceding observation since 2001, we obtained knowledge about planktonic bacterial abundance and their community structure revealed with fluorescence in situ hybridization (FISH). Fluctuation of these numbers is discussed with environmental change as temperature, salinity, phytoplankton abundance and dissolved organic carbon supplied from primary production and terrestrial activities. In particular the population dynamics is chased targeting Vibrio cholerae using specific gene techniques.When compared the whole data set of bacterial abundance with related environments : 1)Temperature showed positive correlation with total bacterial number (P<0.01) and domain Bacteria (P<0.05). 2)Negative relationship with salinity were found for total bacteria (P<0.01), domain Bacteria (P<0.01), gamma -Proteobacteria (P<0.01) and domain Achaea (P<0.05). 3)Abundance of phytoplankton represented by Chl.a showed a higher correlation with domain Bacteria (P<0.01) and that of somewhat lower correlation with gamma-Proteobacteria was then total bacterial number (P<0.05).These findings give us an overview that planktonic bacterial abundance in studied coastal area was governed by fresh water supplied from rivers and it was affected by the shift of temperature though this didn't affect Archaeal abundance. Phytoplankton was then another regulator of bacterial abundance.Vibrio cholerae was chosen a target bacteria which responds to temperature sensitively being a marked harmful bacteria. Method to chase population of cholerae was successfully established, applicable for the studied environment.

2004年至2005年间，我们在骏河湾西水港进行了深入研究。结合2001年以来的前期观测结果，我们获得了浮游细菌丰度及其通过荧光原位杂交（FISH）揭示的群落结构的知识。这些数字的波动随着温度、盐度、浮游植物丰度和初级生产和陆地活动提供的溶解有机碳等环境变化进行讨论。特别是使用特定基因技术针对霍乱弧菌进行种群动态追踪。将整个细菌丰度数据集与相关环境进行比较时：1）温度与细菌总数（P<0.01）和细菌域（P<0.05）呈正相关。）。 2)细菌总数(P<0.01)、细菌域(P<0.01)、γ-变形菌门(P<0.01)和Achaea域(P<0.05)与盐度呈负相关。 3）以叶绿素a为代表的浮游植物丰度与细菌域相关性较高（P<0.01），而与γ-变形菌门相关性较低的是细菌总数（P<0.05）。这些结果使我们可以概述：研究沿海地区的浮游细菌丰度受河流供应的淡水控制，并受到温度变化的影响，尽管这并不影响古菌丰度。浮游植物是细菌丰度的另一个调节因子。霍乱弧菌被选为目标细菌，它对温度敏感，是一种明显的有害细菌。成功建立了适用于所研究环境的霍乱种群追踪方法。