denitrifying factory

反硝化工厂

• 批准号: 23241016
• 负责人: KATO Kenji
• 金额: $ 31.87万
• 依托单位: Shizuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23241016/
• 关键词: 陸圏・水圏・大気圏影響評価; 地下水硝酸汚染

Adequate management of groundwater is strongly required against increasing threat of water shortage. Nitrate contamination is remained problems for groundwater. To minimize nitrate contamination we surveyed nitrate concentration and estimated in situ denitrification activity of groundwater by stable isotope tracer experiment. And we revealed corresponding microbial populations using gene analysis with qPCR targeting denitrification functional genes. Constraints of denitrification are also elucidated. Acetate showed the highest concentration among the measured organic compounds dissolved in the groundwater. And when the groundwater was incubated with 15NO3- by adding acetate debitirification activity increased about twice compared with the incubation under the in situ condition. And soil microbes around the wells enhanced denitrification about three times. Basic understandings were thus obtained to construct in situ highly active denitrification system for the groundwater.

为了应对日益严重的水资源短缺威胁，迫切需要对地下水进行充分的管理。硝酸盐污染仍然是地下水的问题。为了最大限度地减少硝酸盐污染，我们测量了硝酸盐浓度，并通过稳定同位素示踪实验估算了地下水的原位反硝化活性。我们利用针对反硝化功能基因的 qPCR 进行基因分析，揭示了相应的微生物种群。还阐明了反硝化的限制。在测量的地下水中溶解的有机化合物中，乙酸盐的浓度最高。当地下水与15NO3-一起孵育时，通过添加醋酸盐，脱盐活性比原位条件下的孵育增加约两倍。井周围的土壤微生物增强了反硝化作用约三倍。从而对构建地下水原位高活性反硝化系统有了基本认识。

项目成果

Occurrence and potential activity of denitrifiers and methanogens in groundwater at 140 m depth in Pliocene diatomaceous mudstone of northern Japan.

• DOI: 10.1111/1574-6941.12179
• 发表时间: 2013-12
• 期刊: FEMS microbiology ecology
• 影响因子: 4.2
• 作者: Chie Katsuyama;Hiroaki Nashimoto;K. Nagaosa;T. Ishibashi;Kazuki Furuta;T. Kinoshita;H. Yoshikawa;K. Aoki;T. Asano;Yoshito Sasaki;R. Sohrin;D. Komatsu;U. Tsunogai;Hiroyuki Kimura;Y. Suwa;Kenji Kato

Existence and Expression ; from a study on denitrification and methanogenesis in deep subsurface

存在与表达；

• 发表时间: 2011
• 作者: Kenji Kato;Kazuyo Nagaosa
• 通讯作者: Kazuyo Nagaosa

地下圏の脱窒ファクトリー:地下水の硝酸汚染と脱窒(硝酸還元)

地下反硝化工厂：地下水的硝酸盐污染和反硝化（硝酸盐还原）

• 发表时间: 2011
• 作者: 木下岳士;加藤憲二
• 通讯作者: 加藤憲二

浅い地下圏と深い地下圏での脱窒 : 活性とプレイヤー

地下浅层和深层的反硝化：活动和参与者

• 发表时间: 2012
• 作者: 石山絢菜;高治諒;定永靖宗;松木篤;佐藤啓市;長田和雄;坂東博;坂井三郎;寺尾 徹;Nakamura Mariko,;加藤憲二
• 通讯作者: 加藤憲二

Denitrification activity and relevant bacterial community composition in a forest ecosystem.

森林生态系统中的反硝化活性和相关细菌群落组成。

• 发表时间: 2014
• 作者: Chie Katsuyama;Nobuhito Ohte;Yuichi Suwa and Kenji Kato.
• 通讯作者: Yuichi Suwa and Kenji Kato.