Role of miRNAs in the development of bronchial smooth muscle hyperresponsiveness in allergic asthma

miRNA 在过敏性哮喘支气管平滑肌高反应性发展中的作用

• 批准号: 23590118
• 负责人: CHIBA Yoshihiko
• 金额: $ 3.41万
• 依托单位: Hoshi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23590118/
• 关键词: アレルギー性気管支喘息; 気道過敏性; 気管支平滑筋; microRNA (miRNA); miR-140; RhoA; CD38; Interleukin-13 (IL-13); miR-140-5p; TLR4; サイトカイン; interleukin-13 (IL-13)

Current study was carried out to make clear the mechanism(s) of development of airway hyperresponsiveness in asthmatics. In bronchial smooth muscles of mice with allergic asthma, an up-regulation of RhoA protein and a down-regulation of miR-140-3p were observed. In cultured human bronchial smooth muscle cells, an up-regulation of RhoA protein was observed when the function of endogenous miR-140-3p was inhibited by its antagomir. Treatment of the cells with interleukin-13 caused an up-regulation of RhoA protein and a down-regulation of miR-140-3p. These findings suggest that RhoA protein expression is negatively regulated by miR-140-3p in bronchial smooth muscle cells. The increased expression of interleukin-13 in the airways of asthmatics might, at least in part, contribute to the reduction of miR-140-3p, resulting in an up-regulation of RhoA protein and augmented smooth muscle contraction of the airways.

目前的研究是为了清除哮喘患者气道高反应性发展的机制。在患有过敏性哮喘的小鼠支气管平滑肌中，观察到RhoA蛋白的上调和miR-140-3p的下调。在培养的人支气管平滑肌细胞中，当内源性miR-140-3p的功能被其Antagomir抑制时，观察到RhoA蛋白的上调。用白介素13处理细胞会导致RhoA蛋白的上调和miR-140-3p的下调。这些发现表明，在支气管平滑肌细胞中，RhoA蛋白表达受miR-140-3p负调节。白介素13在哮喘患者气道中的表达增加至少可能部分导致miR-140-3p的减少，从而导致RhoA蛋白的上调和呼吸道的增强平滑肌收缩。

项目成果

Elevated guanylate cyclase and cyclic-guanosine monophosphate-dependent protein kinase levels in nasal mucosae of antigen-challenged rats.

• DOI: 10.1016/j.mvr.2013.08.009
• 发表时间: 2013-11
• 期刊: Microvascular research
• 影响因子: 3.1
• 作者: Hiroyasu Sakai;T. Hara;Kenji Todoroki;Yuma Igarashi;M. Misawa;M. Narita;Y. Chiba

アレルギー性気管支喘息時の気管支平滑筋 TLR4 up-regulation における miR-140-5p の役割

miR-140-5p在过敏性支气管哮喘期间支气管平滑肌TLR4上调中的作用

• 作者: 千葉義彦;中原孝史;清水一樹;須藤怜奈;須藤 航;田上 元;團野駿太;加藤靖菜;安藤桂子;松本麻友美;酒井寛泰;成田 年;花崎元彦;片山 浩
• 通讯作者: 片山 浩

Different effects of smoke from heavy and light cigarettes on induction of bronchial smooth muscle hyperresponsiveness in rats

浓烟和淡烟烟雾对诱导大鼠支气管平滑肌高反应性的不同影响

• 发表时间: 2011
• 期刊: J. Smooth Muscle Res
• 作者: Sakai;H.;Fujita;A.;Watanabe;A.;Chiba;Y.;Kamei;J. and Misawa;M
• 通讯作者: M

アレルギー性気管支喘息時の気管支平滑筋 RhoA 発現に及ぼす TSLP の役割

TSLP对过敏性支气管哮喘支气管平滑肌RhoA表达的影响

• 作者: 千葉義彦;須藤 航;清水一樹;須藤怜奈;田上 元;團野駿太;中原孝史;加藤靖菜;安藤桂子;松本麻友美;酒井寛泰;成田 年;花崎元彦;片山 浩
• 通讯作者: 片山 浩

Sphingosine-1-phosphate augments agonist-mediated contraction in bronchial smooth muscles of mice

1-磷酸鞘氨醇增强小鼠支气管平滑肌激动剂介导的收缩

• 发表时间: 2011
• 期刊: Pharmacol. Rep
• 作者: Chiba;Y.;Takeuchi;H.;Sakai;H. and Misawa;M
• 通讯作者: M