Study of diagnosis and spatial epidemiology on paragonimiasis in Lao PDR.

老挝肺吸虫病诊断和空间流行病学研究。

• 批准号: 16390188
• 负责人: NAKAMURA Satoshi
• 金额: $ 8.13万
• 依托单位: Research Institute, International Medical Centre of Japan
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390188/
• 关键词: Infectious disease; Social Medicien; Parasitology; Applied zoology; Ecology; Geographic Information System; 応用動物; ラオス; 肺吸虫症; 診断; 空間疫学; 中間宿主

In spite of lack of information on paragonimiasis in Laos since 1973, eight districts in 5 provinces; Attopeu, Champasak, Savannakhet, Vientiane, and Luangnamtha, which exist and/or possible risk of the disease were clarified through our research. Around 700 metacercariae were collected from 2 species of the river crabs (7 Potamon lipkei and 5 Chulathelphusa brandti) obtained in Hinheup district which was especially investigated as a dense infested area in Vientiane province, where is 80 km north from Vientiane Capital in Lao PDR. Though the numbers were smaller, Paragonimus heterotremus, a known causative species of human parogonimiasis in Indochina region, and P. westermani were identified at the metacercariae population. On the major population, 2 unreported Paragonimus species of harinastai and bankokensis were identified and clarified using adult worms obtained from experimental infections of dogs in the laboratory. Among various experimental animals, adult worms of P. harinastai never survive after 3 month of the experimental infections in rats, which was demonstrated for the first time. Furthermore, dog is a suitable host for P. bankokensis was confirmed by recovery (80%) of the adult worms at the experimental infections. And the established PCR technology was used. To identify the actual causative species of human paragonimiasis in Hinheup, 6 samples of the parasite eggs from the sputa of dwellers were collected, and the established PCR technique; using primers of species specific region (ITS2) of the 4 parasites, was performed. All these samples resulted in P. heterotremus, thus the causative parasite of human paragonimiasis was strongly suggested as this species in the region.

尽管自 1973 年以来老挝缺乏关于肺吸虫病的信息，但 5 个省的 8 个地区；通过我们的研究，澄清了Attopeu、Champasak、Savannakhet、Vientiane 和Luangnamtha 等地区存在和/或可能存在该疾病风险的地区。从老挝人民民主共和国万象首都以北 80 公里的万象省 Hinheup 地区获得的 2 种河蟹（7 只 Potamon lipkei 和 5 只 Chulathelphusa Brandti）中收集到约 700 个囊蚴。尽管数量较少，但在囊蚴种群中发现了异震并殖吸虫（印度支那地区已知的人类并殖吸虫病病原体）和韦氏并殖吸虫。在主要种群中，利用实验室感染狗的成虫，鉴定并澄清了 2 种未报告的并殖吸虫属 harinastai 和bankokensis。在各种实验动物中，首次证实P. harinastai成虫在大鼠实验感染3个月后从未存活。此外，通过实验感染时成虫的回收率（80％）证实狗是 P.bankokensis 的合适宿主。并使用了已建立的PCR技术。为鉴定兴和市人类肺吸虫病的实际致病种类，采集了6份居民痰液中的寄生虫卵样本，并建立了PCR技术；使用 4 种寄生虫的物种特异性区域 (ITS2) 的引物进行了检测。所有这些样本都发现了异旋肺吸虫，因此强烈建议该地区的该物种是人类肺吸虫病的致病寄生虫。

项目成果

Distribution of Paragonimus species in the crab host at Vientiane Capital in Lao People's Democratic Republic

老挝人民民主共和国首都万象蟹寄主中并殖吸虫属的分布

• 作者: Satoshi Nakamura; Shigehisa Habe; et al.
• 通讯作者: et al.

ラオスにおける人肺吸虫症の原因種の同定(学会発表)

老挝人类肺吸虫病病原体的鉴定（会议报告）

• 发表时间: 2006
• 作者: 波部重久; 八尋伸一郎; 中村 哲
• 通讯作者: 中村 哲

ラオスにおける肺吸虫症

老挝并殖吸虫病

• 发表时间: 2005
• 作者: 波部重久; 中村 哲; 他
• 通讯作者: 他

秋道智彌 編 図説 メコンの世界

富宫秋道编 湄公河世界插画

• 发表时间: 2007
• 作者: 中村 哲

Identification of human Paragonimus species collected from Laotian patients.

从老挝患者身上采集的人类并殖吸虫种类的鉴定。

• 发表时间: 2006
• 作者: S.Yahiro; S.Habe; S.Nakamura et al.
• 通讯作者: S.Nakamura et al.