Clarification of mechanisms for hierarchical gene expression in RNA world and its application

RNA世界中分层基因表达机制的阐明及其应用

• 批准号: 21689049
• 负责人: MORI Ryoichi
• 金额: $ 16.81万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21689049/
• 关键词: 免疫; 炎症; microRNA; 次世代シークエンサー; 皮膚創傷治癒; miRNA; 組織修復; アンチセンスオリゴ; 線維化; DNAアレイ; 次世代シーケンサー

Inflammation-related miRNAs might be involved in regulation of inflammatory response and tissue fibrosis. However, these genes have not been identified yet. In the present study, we identified wound-related known and unknown miRNAs using next generation sequencing. Then we identified inflammation-related miRNAs and clarified its functions using histological, cellular and molecular biological, and imaging analysis in vivo and in vitro.

炎症相关的 miRNA 可能参与炎症反应和组织纤维化的调节。然而，这些基因尚未被识别。在本研究中，我们使用下一代测序鉴定了与伤口相关的已知和未知 miRNA。然后我们利用组织学、细胞和分子生物学以及体内和体外成像分析鉴定了炎症相关的 miRNA 并阐明了其功能。

项目成果

Identification of differentially expressed microRNA in blood plasma of calorie restricted and obese mice by next generation sequencing

通过二代测序鉴定热量限制小鼠和肥胖小鼠血浆中差异表达的 microRNA

• 发表时间: 2010
• 作者: Gruz Petr;Masatomi Shimizu;Mori R
• 通讯作者: Mori R

Development of a bioassay to screen for chemicals mimicking the anti-aging effects of calorie restriction

• DOI: 10.1016/j.bbrc.2010.09.032
• 发表时间: 2010-10-15
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Chiba, Takuya;Tsuchiya, Tomoshi;Shimokawa, Isao
• 通讯作者: Shimokawa, Isao

Acute stress response modified by modest inhibition of growth hormone axis : apotential machinery of the anti-aging effect of calorie restriction

通过适度抑制生长激素轴来改变急性应激反应：热量限制的抗衰老作用的潜在机制

• DOI: 10.1016/j.mad.2011.01.002
• 发表时间: 2011
• 期刊: Mechanisms of Ageing and Development
• 影响因子: 5.3
• 作者: Komatsu T;Trindade LS;Chiba T;Hayashi H;Henmi T;Ushiroda Y;Mori R;Shimokawa I
• 通讯作者: Shimokawa I

Molecular mechanisms of cytokine secretion in macrophages

巨噬细胞分泌细胞因子的分子机制

• 发表时间: 2009
• 作者: 森亮一;池松和哉;下川功;坪井貴司
• 通讯作者: 坪井貴司

Molecular mechanisms of cytokine secretion in macrophage

巨噬细胞分泌细胞因子的分子机制

• 发表时间: 2009
• 作者: 河原貴展、山下元三、橋本悠平;中村友美;梶川哲宏;前田憲一郎;北垣次郎太;山田聡;北村正博;村上伸也;森亮一
• 通讯作者: 森亮一