Laminar Bone Foromation and Comparative Histology on Osteon-Remodeling in Some Species of Aritidactla

一些拟南芥属的板层骨形成和骨重塑的比较组织学

基本信息

批准号：
09670030
负责人：
MORI Ryoichi
金额：
$ 1.15万
依托单位：
Showa University School of Dentistry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670030/
关键词：
Artiodactyla laminar bone bright line woven bone lamellar bone appearance of osteon comparative histology scanning electron microscopy 歯状骨四肢長骨組織学走査電顕・分析電顕骨形成ハバース層板骨

项目摘要

We investigated the limb long-bone diaphyses of various-aged bovines, goats, pigs, and sheep by transmitted light, scanning electron, transmitted elect'ron microscopy, and enegy-dispersive X-ray microanalysis.In a 3-rnonthfetus bovine, 3-4 concentric layers of laminar bone appeared around the bone marrow and 25-26 layers were seen in a new-born calf. In a 1-year-old ox, osteons appeared in the inner-third zone, however, the laminar bone was retained yet in a 12-year-old cow. The hypercalcified center of laminar bone, so-called a bright line, scattered relatively large granular and fine fibrillar structures. After formation of this line, woven bone and lamellar bone were formed on the line. The appositional volume was larger in the endosteum than the periosteum side. There were a few osteocytes in the bright line, but the cell lacunae were larger than the normal lacunae. Thus, the formation of bone matrix may somewhat differ from each other. From the bright line, larger amounts of Na and S elements were detected.In Van-Gieson staining for collagen fibrils, the bright line showed no staining. In H-E and AZAN staining, the line was distinguishable from the woven and lamellar bones, and also differed from them in the younger laminar bone stained with toluidin blue. However, we can not identified the organic elements of the granular and fibrillar structures found in the bright line.In the formation of limb-long bones in young goats, pigs, and sheep, laminar bone similar to that of bovines was also observed. The laminar bone was rapidly formed and osteons that should form Haversian bone did not appear in the laminar bone for a certain period of time after birth. The appearance was earlier seen in pigs and latest in bovines as a large animal.

我们通过透射光、扫描电子、透射电子显微镜和能量色散 X 射线微量分析研究了不同年龄的牛、山羊、猪和绵羊的四肢长骨骨干。在 3 胎牛中，3骨髓周围出现-4层同心层状骨，在新生犊牛中看到25-26层。 1岁牛的内三分之一区已出现骨骨，而12岁牛的板骨尚未保留。板层骨中心过度钙化，即所谓的亮线，散布着较大的颗粒状和细纤维状结构。该线形成后，在线上形成编织骨和板层骨。内膜侧的并置体积大于骨膜侧。亮线内有少量骨细胞，但细胞陷窝较正常陷窝大。因此，骨基质的形成可能彼此有所不同。从亮线中，检测到大量的Na和S元素。在胶原原纤维的Van-Gieson染色中，亮线显示没有染色。在H-E和AZAN染色中，该线与编织骨和板层骨不同，并且在用甲苯胺蓝染色的较年轻的板层骨中也与它们不同。然而，我们无法识别亮线中发现的粒状和纤维状结构的有机元素。在幼山羊、猪和绵羊的肢长骨的形成中，也观察到与牛相似的层状骨。板层骨迅速形成，出生后一定时间内板层骨中没有出现应形成哈弗氏骨的骨。这种现象较早出现在猪身上，最近出现在大型动物牛身上。