The qualitative and quantitative analysis of olfactory glomerulus and nidus in the olfactory bulb

嗅球内嗅球和巢的定性和定量分析

• 批准号: 13680823
• 负责人: KOSAKA Katsuko
• 金额: $ 2.37万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680823/
• 关键词: Anatomy; Cell Tissue; Neuroscience; Brain Neuron; Confocal laser microscopy

We investigated immunohistochemically the main olfactory bulbs (MOBs) of 7 mammalian species, focusing on two points: 1) whether nidi, particular spheroidal synaptic regions containing many GABAergic calbindin D28K-immunoreactive (CB-IR) elements we previously reported in the laboratory musk shrew MOBs, are also present in other animals, and 2) whether the compartmental organization of glomeruli and two types of periglomerular cells we proposed in the rat MOBs, are general in the MOBs, of these animals. We used two insectivores, moles and hedgehogs, tree shrews, bats and mice as well as laboratory musk shrews and rats. We found discrete nidi and the nidal layer only in the mole and laboratory shrew MOBs, where observed none or a few olfactory nerves (Ons) extruding from glomeruli. These results suggest that the nidi and related distinctive organization might be specific to only some species of insectivores.On the other hand, we recognized the apparent compartmental organization of glomeruli and two types of periglomerular cells in the MOBs of all of the mammals we examined, indicating these structural features are common to the MOBs of various mammals and thus might be important organizational principles of the MOBs.

我们通过免疫组织化学方法研究了 7 种哺乳动物的主要嗅球 (MOB)，重点关注两点：1) 巢状突触区域是否含有我们之前在实验室麝香鼩中报道的许多 GABA 能钙结合蛋白 D28K 免疫反应 (CB-IR) 元件MOB 也存在于其他动物中，2) 肾小球的区室组织和两种类型是否我们在大鼠 MOB 中提出的肾小球周围细胞在这些动物的 MOB 中很常见。我们使用了两种食虫动物：鼹鼠和刺猬、树鼩、蝙蝠和小鼠以及实验室麝香鼩和大鼠。我们仅在鼹鼠和实验室鼩鼱 MOB 中发现了离散的巢穴和巢穴层，在这些 MOB 中观察到没有或有少量嗅觉神经 (Ons) 从肾小球伸出。这些结果表明，鸟巢和相关的独特组织可能仅针对某些食虫动物物种。另一方面，我们在我们检查的所有哺乳动物的 MOB 中认识到肾小球的明显区室组织和两种类型的肾小球周围细胞，表明这些结构特征是各种哺乳动物的 MOB 所共有的，因此可能是 MOB 的重要组织原则。

项目成果

K.Kosaka, T.Kosaka: "Nidus and Tasseled cell : Distinctive neuronal organization of the main olfactory bulb of the laboratory musk shrew (Suncus murinus)"Journal of Comparative Neurology. 430. 542-561 (2001)

K.Kosaka，T.Kosaka：“巢和缨细胞：实验室麝鼩（Suncus murinus）主要嗅球的独特神经元组织”比较神经病学杂志。

Kosaka T., Kosaka K: "Neuronal gap junctions in the rat main olfactory bulb, with special reference to intraglomerular gap junctions"Neuroscience Research. 45(2). 189-209 (2003)

Kosaka T.、Kosaka K：“大鼠主嗅球中的神经间隙连接，特别是肾小球内间隙连接”神经科学研究。

Kosaka T., Kosaka K.: "Neuronal gap junctions in the rat main olfactory bulb, with special reference to intraglomerular gap junctions"Neuroscience Research. 45:2. 189-209 (2003)

Kosaka T.、Kosaka K.：“大鼠主嗅球中的神经间隙连接，特别是肾小球内间隙连接”神经科学研究。

Kosaka K., Akita Y., Toida K., Kosaka T.: "Structure of intraglomerular dendritic tufts of mitral cells and their contacts with olfactory nerve terminals and calbindin-immunoreactive type 2 periglomerular neurons"J. Comp. Neurol.. 440. 219-235 (2001)

Kosaka K.、Akita Y.、Toida K.、Kosaka T.：“二尖瓣细胞肾小球内树突簇的结构及其与嗅觉神经末梢和钙结合蛋白免疫反应性 2 型肾小球周围神经元的接触”J。

Kosaka K., Kosaka T: "Nidus and tasseled cell : Distinctive neuronal organization of the main olfactory bulb of the laboratory musk shrew (Suncus murinus)"J.Comp.Neurol. 430. 542-561 (2001)

Kosaka K.，Kosaka T：“巢和缨细胞：实验室麝鼩（Suncus murinus）主要嗅球的独特神经元组织”J.Comp.Neurol。