Comparative Analyses of the Main Olfactory Bulb : Rodent and Insectivore

主要嗅球的比较分析：啮齿动物和食虫动物

基本信息

批准号：
18500265
负责人：
KOSAKA Katsuko
金额：
$ 2.52万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

项目摘要

Glomeruli of the main olfactory bulb (MOB) are now considered to serve as functional units in the olfactory information processing. We confirmed the compartmental organization of the glomerulus and the presence of both type 1 and 2 periglomerular cells in the mice. Similar to rat periglomerular cells, the tyrosine hydroxylase (TH) -positive cells were type 1 and GAD/GABA-positive. On the other hand, both the calbindin D28k (CB) -positive and calretinin (CR) -positive cells were type 2 periglomerular cells, but in contrast to those in rats, which are GAD/GABA-negative, all of the calbindin D28k-positive periglomerular cells and 50% of the calretinin-positive periglomerular cells were GAD/GABA-positive. The GAD/GABA-positive cells thus included both type 1 and type 2 periglomerular cells. Juxtaglomerular NOS-positive cells have been proposed as a subgroup of type 1 periglomerular cells that are separate from the CR-positive and CB-positive cells in rats. However, in the mice, about 70% of the NOS-positive cells were calretinin-positive, and 50% of the CR-positive cells were NOS-positive. We herein reveal the significant species differences in the chemical properties of periglomerular cells and suggest that the cellular organization of the mouse main olfactory bulb cannot be extrapolated from that of rats.Furthermore, we reported the precise distribution and characteristic features of NOS-positive and PV-positive neurons in the mouse MOB.

主嗅球（MOB）的肾小球现在被认为是嗅觉信息处理中的功能单位。我们证实了小鼠肾小球的区室组织以及 1 型和 2 型肾小球周围细胞的存在。与大鼠肾小球周围细胞类似，酪氨酸羟化酶 (TH) 阳性细胞为 1 型且 GAD/GABA 阳性。另一方面，钙结合蛋白 D28k (CB) 阳性和钙结合蛋白 (CR) 阳性细胞均为 2 型肾小球周围细胞，但与 GAD/GABA 阴性的大鼠中的细胞相反，所有钙结合蛋白 D28k-阳性肾小球周围细胞和 50% 的钙结合蛋白阳性肾小球周围细胞为 GAD/GABA 阳性。因此，GAD/GABA 阳性细胞包括 1 型和 2 型肾小球周围细胞。肾小球旁 NOS 阳性细胞被认为是 1 型肾小球周围细胞的一个亚群，与大鼠中的 CR 阳性和 CB 阳性细胞分开。然而，在小鼠中，约70%的NOS阳性细胞是钙结合蛋白阳性，50%的CR阳性细胞是NOS阳性。我们在此揭示了肾小球周围细胞化学性质的显着物种差异，并表明小鼠主嗅球的细胞组织不能从大鼠的细胞组织中推断出来。此外，我们报告了 NOS 阳性和 PV 的精确分布和特征-小鼠MOB中的阳性神经元。