Morphological Analyses of the Glomerulus in the Main Olfactory Bulb.

主嗅球肾小球的形态分析。

• 批准号: 15300115
• 负责人: KOSAKA Katsuko
• 金额: $ 9.47万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15300115/
• 关键词: Olfactory bulb; interneuron; GABA; gap junction; synapse; confocal laser scanning microscope; electron microscope; 介在ニューロン; 主ニューロン; 免疫細胞化学; レーザー顕微鏡; 嗅覚伝達

Glomeruli of the main olfactory bulb (MOB) are now considered to serve as functional units in the olfactory information processing. Our previous analyses of the rat MOB revealed the compartmental organization of glomeruli and two types of periglomerular (PG) cells, type 1 (PG1)and type 2 (PG2) PG cells. We found that olfactory nerve axon terminals made synaptic contacts with dendritic tufts of both projection neurons (mitral and tufted cells) and PG1, but not of PG2, cells in the rat MOB. In the present project we extended our investigation to the fine structural organization of the glomeruli of mice (including genetically manipulated mice) and other mammals. Using the confocal laser scanning microscope and electron microscope, we found that the colocalization relationship of chemical substances in mouse PG cells was different from that of the rat PG cells reported previously.Furthermore, we confirmed the compartmental organization of the glomeruli and the presence of two types of PG cells in the MOB of bats, tree shrews, hedgehogs, moles, musk shrews and tenrecs. These structural features are therefore considered to be common and important organizational principles of the MOBs. We also exhibited complex neuronal interactions via chemical synapses and gap junctions in the glomerulus.

主嗅球（MOB）的肾小球现在被认为是嗅觉信息处理中的功能单位。我们之前对大鼠 MOB 的分析揭示了肾小球的区室组织和两种类型的肾小球周围 (PG) 细胞，即 1 型 (PG1) 和 2 型 (PG2) PG 细胞。我们发现嗅神经轴突末梢与大鼠 MOB 中投射神经元（二尖瓣和簇状细胞）和 PG1 细胞的树突簇发生突触接触，但不与 PG2 细胞发生突触接触。在本项目中，我们将研究扩展到小鼠（包括基因操纵小鼠）和其他哺乳动物肾小球的精细结构组织。利用共聚焦激光扫描显微镜和电子显微镜，我们发现小鼠PG细胞中化学物质的共定位关系与之前报道的大鼠PG细胞不同。此外，我们证实了肾小球的区室组织以及两种物质的存在。蝙蝠、树鼩、刺猬、鼹鼠、麝鼩和马岛猬的 MOB 中 PG 细胞的类型。因此，这些结构特征被认为是 MOB 的共同且重要的组织原则。我们还通过肾小球中的化学突触和间隙连接展示了复杂的神经元相互作用。

项目成果

Structural organization of the glomerulus in the main olfactory bulb.

主嗅球中肾小球的结构组织。

• 发表时间: 2005
• 作者: Kosaka T; Kosaka K
• 通讯作者: Kosaka K

Neuronal gap junctions in the rat main olfactory bulb, with special reference to intraglomerular gap junctions.

大鼠主嗅球中的神经元间隙连接，特别是肾小球内间隙连接。

• 发表时间: 2003
• 作者: Kosaka; T.; Kosaka; K.
• 通讯作者: K.

匂いと香りの科学(澁谷達明、市川真澄 編著)

气味与香味的科学（涉谷龙明、市川真澄编）

• 发表时间: 2005
• 作者: 小坂俊夫; 小坂克子
• 通讯作者: 小坂克子

Intraglomerular dendritic link connected by gap junctions and chemical synapses in the mouse main olfactory bulb : electron microscopic serial section analyses.

小鼠主嗅球中通过间隙连接和化学突触连接的肾小球内树突连接：电子显微镜系列切片分析。

• 发表时间: 2005
• 作者: Kosaka T; Kosaka K.
• 通讯作者: Kosaka K.

Neuronal gap junctions between intraglomerular mitral/tufted cell dendrites in the mouse main olfactory bulb.

小鼠主嗅球肾小球内二尖瓣/簇状细胞树突之间的神经元间隙连接。

• 发表时间: 2004
• 作者: Kosaka; T.; Kosaka; K.
• 通讯作者: K.