Acquisition of a Bruker 11.7T/16cm Preclinical Scanner for Novel MRI/MRSI Studies

采购布鲁克 11.7T/16cm 临床前扫描仪用于新型 MRI/MRSI 研究

基本信息

批准号：
10630511
负责人：
Dewan Syed Fahmeed Hyder
金额：
$ 200万
依托单位：
YALE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-15 至 2024-09-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10630511
关键词：
20 year old 3-Dimensional Advisory Committees Anatomy Animals Brain Brain imaging Cell Nucleus Communities Cortical Column Development Disease Education Educational process of instructing Extramural Activities Faculty Failure Functional Imaging Funding Goals Helium Human Institution Magnetic Resonance Magnetic Resonance Imaging Mentors Metabolic Minor Neurosciences Research Nuclear Organoids Pathogenesis Performance Postdoctoral Fellow Pump Rat Transgene Rattus Research Research Personnel Research Project Grants Resolution Rodent Spectrum Analysis Study models System Technical Expertise Technology Transgenic Mice Transgenic Model Transgenic Organisms Translations United States National Institutes of Health Universities cost cryogenics experience graduate student improved magnetic resonance spectroscopic imaging metabolic imaging method development multimodality novel operation peer pre-clinical spectroscopic imaging structural imaging tissue fixing translation to humans translational model translational neuroscience ultra high resolution

项目摘要

Transgenic rodents are the most important translational model for studying pathogenesis and treatment of disease. However magnetic resonance imaging (MRI) and spectroscopy (MRS) as well as spectroscopic imaging (MRSI) studies of transgenic mouse and recently transgenic rat have been limited due to not having sufficient spatial resolution to distinguish key anatomic structures such as cortical columns and layers. With development of ultrahigh field (UHF) animal MR systems, using cryogenic technology for sensitivity enhancement, there is unique opportunity to break this sensitivity barrier allowing ultrahigh resolution functional, structural, and metabolic 3D brain imaging in transgenic models, with a direct path to translation to human studies. The primary goal of this proposal is to install and develop capabilities of a state-of-the-art UHF Bruker 11.7T/16cm preclinical scanner with cryoprobes for 1H and X nuclei at Yale's Magnetic Resonance Research Center (MRRC) for ultrahigh resolution functional, structural, and metabolic imaging in small animals, fixed tissues, and human organoids. Our primary Justification of Need is that this new system will replace an unsupported 20-year old 11.7T pumped magnet with extremely high helium costs that is close to failure. The new 11.7T system will allow our outstanding userbase, and investigators throughout the NIH community, to continue their funded research on a reliable state-of-the-art system, and obtain several-fold sensitivity gains for multi-modal MRI/MRSI via cryogenic coil technology and to answer cutting edge basic and translational neuroscience research questions. The system will be managed by a team of MRI/MRSI researchers with outstanding Technical Expertise and decades of track record in operating and developing UHF systems for MRI/MRSI studies. We have identified 14 Major Users with 18 NIH Research Projects that will most benefit from the new system along with 8 Minor Users. Several are from peer institutions who are performing their preclinical MRI/MRSI projects at Yale MRRC due to our unique method developments. The Administration plan builds on our 35-year experience in facility management, with highly experienced operations, steering, and advisory committees with leading intramural and extramural researchers. Yale University will provide a substantial Institutional Commitment totaling $2,100,000 in matching funds towards the system's cost and pay for complete installation. The system will also benefit Trainee Education based on participation of many of the core MR faculty in mentoring of graduate students and post-doctoral fellows, as well as classroom teaching. By combining UHF with superior gradient performance and cryoprobes for 1H and X nuclei we anticipate an approximately 2.5- to 4-fold gain in sensitivity over our existing unsupported 11.7T system. These improvements will allow translation of our multinuclear and multimodal MRI/MRSI to studies of the transgenic mouse brain and ultrahigh resolution rat brain studies. Thus, the requested 11.7T will fulfill urgent needs for state-of-the-art MRI/MRSI research for NIH-funded investigators at Yale, and elsewhere.

转基因啮齿动物是研究发病机制和治疗的最重要的转化模型疾病。然而，磁共振成像 (MRI) 和光谱学 (MRS) 以及光谱学转基因小鼠和最近转基因大鼠的成像（MRSI）研究由于没有足够的空间分辨率来区分关键的解剖结构，例如皮质柱和皮质层。和开发超高场 (UHF) 动物 MR 系统，使用低温技术提高灵敏度增强，有独特的机会打破这种灵敏度障碍，从而实现超高分辨率转基因模型中的功能、结构和代谢 3D 脑成像，可直接转化为人类研究。该提案的主要目标是安装和开发最先进的功能 UHF Bruker 11.7T/16cm 临床前扫描仪，配有耶鲁磁共振 1H 和 X 核冷冻探针研究中心 (MRRC)，用于小型超高分辨率功能、结构和代谢成像动物、固定组织和人类类器官。我们的主要需求理由是这个新系统将更换无支撑的 20 年旧 11.7T 泵磁体，氦气成本极高，接近失败。新的 11.7T 系统将使我们优秀的用户群和整个 NIH 的研究人员社区，继续资助研究可靠的最先进的系统，并获得数倍的回报通过低温线圈技术提高多模态 MRI/MRSI 的灵敏度，并回答尖端的基本和转化神经科学研究问题。该系统将由 MRI/MRSI 团队管理拥有杰出技术专长和数十年运营和开发经验的研究人员用于 MRI/MRSI 研究的 UHF 系统。我们已经确定了 14 个主要用户和 18 个 NIH 研究项目，将与 8 个小用户一起从新系统中受益最多。其中有几位来自同行机构由于我们独特的方法开发，他们在耶鲁 MRRC 执行临床前 MRI/MRSI 项目。这管理计划建立在我们 35 年的设施管理经验之上，我们拥有经验丰富的人员由校内和校外主要研究人员组成的运营、指导和咨询委员会。耶鲁大学大学将提供总计 2,100,000 美元的大量机构承诺，作为配套资金系统的成本和完整安装的费用。该系统还将有利于学员教育许多 MR 核心教师参与研究生和博士后研究员的指导，如还有课堂教学。通过将 UHF 与卓越的梯度性能和 1H 和我们预计 X 核的灵敏度比我们现有的无支持的 11.7T 提高约 2.5 至 4 倍系统。这些改进将使我们的多核和多模态 MRI/MRSI 转化为以下研究：转基因小鼠大脑和超高分辨率大鼠大脑研究。因此，所要求的11.7T将满足耶鲁大学和其他地方的 NIH 资助的研究人员迫切需要最先进的 MRI/MRSI 研究。