Role of plasma S19 ribosomal protein in thrombus resorption

血浆S19核糖体蛋白在血栓吸收中的作用

基本信息

批准号：
18590374
负责人：
YAMAMOTO Tetsuro
金额：
$ 2.57万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18590374/
关键词：
S19 ribosomal protein coagulation factor XIII monocyte macrophage leukocyte chemotactic factor thrombus blood coagulation cellular fibrinolysis 単球走化因子細胞性線溶現象

项目摘要

To elucidate roles of S19 ribosomal protein present in plasma and monocytes in the thrombus resorption, we developed an experimental model in which pre-formed blood coagula was transplanted into the guinea pig peritoneal cavity. The surface of the coagula was fully covered by monocytes/macrophages within a day after the transplantation. The monocytes/macrophages on the coagula inter-connected each other forming an epithelia-like pattern. The size of coagula rapidly decreased by day 3 and disappeared by day 7.We raised anti-S19 ribosomal protein antibodies in rabbits. When the pre-formed blood coagula was centrifuged and serum was obtained, a monocyte-directed chemotactic factor was recovered in the serum. The monocyte chemotactic factor was immunologically identified to be the S19 ribosomal protein dimer. When guinea pig blood was coagulated under the presence of the anti-S19 ribosomal protein antibodies and then transplanted into the peritoneal cavity, all of the above events in the resorption process were significantly reduced. Especially, the coagula surface was covered only partly by monocytes/macrophages and the epithelia-like formation of these cells was not seen.These results strongly suggested that the S19 ribosomal protein in plasma was cross-linked by activated coagulation factor XIII resulting in the dimer formation during blood coagulation and that this dimer recruited monocytes which would involve in the coagula resorption.

为了阐明存在于血栓吸收中血浆和单核细胞中的S19核糖体蛋白的作用，我们开发了一个实验模型，在该模型中，将预先形成的血液凝结物移植到豚鼠腹膜腔中。移植后一天的一天，凝血菌的表面被单核细胞/巨噬细胞完全覆盖。凝结物上的单核细胞/巨噬细胞相互关联，形成了类似上皮的模式。和瓜拉的大小在第3天之前迅速减少，并在第7天消失。我们在兔子中升高了抗S19核糖体蛋白抗体。当离心预形成的血液凝结物并获得血清时，在血清中回收了单核细胞指导的趋化因子。单核细胞趋化因子在免疫学上被鉴定为S19核糖体蛋白二聚体。当豚鼠血液在抗S19核糖体蛋白抗体的存在下凝结，然后将其移植到腹膜腔中时，吸收过程中的所有上述事件都显着降低。尤其是，尤其是仅通过单核细胞/巨噬细胞部分覆盖凝结物表面，并且没有看到这些细胞的上皮样形成。这些结果强烈表明，血浆中的S19核糖体蛋白通过激活的凝血因子XIII在二凝结中形成二凝结和该二聚体的繁殖物在二聚体过程中产生的二聚体形成，从而在多种二聚体中进行了繁殖。