Studies on the membrane properties of the cat parietal cortical pyramidal neurons concerning motor compensation

猫顶叶皮层锥体神经元运动补偿膜特性的研究

基本信息

批准号：
06680808
负责人：
YAMAMOTO Tetsuro
金额：
$ 1.41万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06680808/
关键词：
INTRACELLULAR RECORDING INTRACELLULAR STAINING CEREBRAL CORTEX IN VITRO SLICE PREPARATION EPSP MOTOR COMPENSATION FACIAL NUCLEUS NEURONAL CIRCUIT IN THE BRAINSTEM 視床-皮質投射

项目摘要

The aims of this project are to investigate the electrophysiological and morphological substrates for the neural plasticity concerning the motor compensation. During the research project, we investigated the three aspects of the neuronal system. First, we examined the membrane properties of cortical neurons from slice preparations of the cat sensorimotor cortex and the parietal cortex. Second, we studied the changes of the cerebello-parietal projection after ablation of the motor cortex. Third, we revealed the time course and synaptic plasticity after the facial nerve suture. At the end of the project, we obtained several results on these three projects. Concerning slice experiments, we found that the burst firing neurons exist in the layr III of the sensorimotor cortex. When applying high frequency stimulation (tetanus stimuli) onto the white matter, layr III and V pyramidal neurons displayd long term potentiation or depression with membrane potential changes in either direction. Such synaptic plasticity may not be concerned with NMDA receptors. In the second project, we must continue the experiment, beacause the constant changes of the cerebello-parietal responses could not be obtained after the motor cortical ablation. However, in the third project, we reported the early regeneration of the sutured facial nerve. The functional recovery examined by the facial muscle EMG was detected as early as 11 days after suture. During the regeneration of the sutured facial nerve, we also found that the trigemino-facial reflex showed transient change in 1-2 weeks after operation, i.e., the reflex nerve discharges were induced not only in the main branch as normal but also in the posterior-auricular branch. Such a transient change of the reflex pattern returned normal in 3 weeks after suture. We are now planning to examine the details in such a change electrophysiologically as well as morphologically.

该项目的目的是研究有关运动补偿的神经可塑性的电生理和形态底物。在研究项目中，我们研究了神经元系统的三个方面。首先，我们检查了CAT感觉运动皮质和顶叶皮质的切片制剂中皮质神经元的膜特性。其次，我们研究了运动皮层消融后小脑 - 长方面投射的变化。第三，我们揭示了面神经缝合线后的时间过程和突触可塑性。在项目结束时，我们在这三个项目上获得了几个结果。关于切片实验，我们发现在感觉运动皮层的Layr III中存在爆发的神经元。当将高频刺激（破伤风刺激）应用于白质时，Layr III和V锥体神经元显示长期增强或抑郁，并在任一方向上具有膜电位变化。这种突触可塑性可能与NMDA受体无关。在第二个项目中，我们必须继续进行实验，标志着运动皮质消融后无法获得小脑长方体反应的恒定变化。但是，在第三个项目中，我们报告了缝合面神经的早期再生。在缝合线11天后，检测到由面部肌肉EMG检查的功能恢复。在缝合的面神经的再生过程中，我们还发现，在手术后1-2周内，三角骨群落反射显示出瞬态变化，即，反射神经放电不仅在正常的主分支中诱导，而且在后骨分支中也被诱导。这种反射模式的这种短暂变化在缝合后3周内恢复了正常状态。我们现在计划在电生理学以及形态学上进行这种变化的细节。