Periodontal tissue regeneration method by regenerative medicine with stem cells-multi-center study for practical use

干细胞再生医学牙周组织再生方法-多中心实用化研究

• 批准号: 16390583
• 负责人: UEDA Minoru
• 金额: $ 9.15万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390583/
• 关键词: regenerative medicine; mesenchymal stem cells; periodontal tissue regeneration; clinical application; practical use; platelet rich plasma; 歯周組織再生

Periodontal disease is an infectious disease affecting tooth-supporting tissues. But in this present, effective treatment for the periodontal disease is not. On the other hand, little regenerative medicine is applied for practical use. In this study, the regenerative medicine technology is applied for periodontal disease. We used PRP as an autologous scaffold-which provides signal molecules-with in vitro-expanded mesenchymal stem cells (MSCs), and called this method 'injectable tissue engineerd bone (T.E.B.)'. As basic research we investigated about the ability of periodontal tissue regeneration by T.E.B. regeneration in a dog mandible periodontal model. The results showed that epithelial tissue down growth is few in T.E.B. (dogMSCs/PRP) and T.E.B. and GTR method compared to control (flap operation) in histological, histomorphometric observations, and that the MSCs in the T.E.B. regenerated new cementum the same as a natural cementum. Secondaly before clinical approach we investigated … More the characteristic and safety of the MSCs by cell surface antibody with FACS, mycoplasma infection, endtoxin, gene mutation, tumor formation test and did not find abnormal condition. After the research protocol was approved by the University Ethics Committee, we discussed about the operation method and adaptation case by multi-center and applied for clinical approach. It did bone marrow biopsy 1 month and half, and prepared 1 day before operation. The culture process did under sterile condition for practice use. Before operation the patient did plaque control and periodontal initial treatment. From the result of animal experiment we did not use membrane using by GTR method in this clinical application. The result in clinical application published in Int J Periodont Rest, 26, 363-369, 2006. The post-operation process is good and it showed the ability of true periodontal regeneration for periodontitis by MSCs. In the future, we would like to establish novel periodontal tissue regeneration method with minimal invasiveness by translational research concept and contribute improvement in quality of life (QOL) for the patients. Less

牙周疾病是一种影响牙齿支持的组织。自体内的分子在体外膨胀的间充质细胞CS，并将这种方法称为“可注射的组织工程骨（T.E.B.）”导致在T.E.B.（DogMSCS/PRP）和T.E.B和GTR方法中降低质量，与组织学，组织学，组织学观察结果相比（皮瓣操作）细胞表面抗体的安全性，具有FASS的细胞抗体，支原体感染，末端毒素，基因突变，肿瘤形成测试，并未发现多个中心的适应性。一半和一半的培养过程中的培养过程是练习的。周期性休息，26，363-369，2006。在未来使用MSC的牙周炎，我们想建立新的牙周组织再生方法，并通过翻译研究概念建立新的牙周组织再生方法。并促进生活质量的改善

项目成果

注入型培養骨を用いた歯周組織再生

使用可注射培养骨进行牙周组织再生

• 发表时间: 2004
• 期刊: 化学工業 55・7
• 作者: Yamada;Y.;Ueda;M.;et al.;大屋 盛道 ほか;山田 陽一 ほか;山田 陽一 ほか;山田 陽一 ほか
• 通讯作者: 山田 陽一 ほか

間葉系幹細胞を用いた骨の再生医療

使用间充质干细胞的骨再生医学

• 发表时间: 2006
• 期刊: 日本老年医学会雑誌 43・5
• 作者: 山田陽一;上田実
• 通讯作者: 上田実

Translational research for injectable tisuue-engmeered bone regeneration using mesenchymal stem cells and platelet-rich plasma - from basic research to clinical case study -

使用间充质干细胞和富含血小板的血浆进行可注射组织工程骨再生的转化研究 - 从基础研究到临床案例研究 -

• 发表时间: 2004
• 期刊: Cell Transplant. 13
• 作者: Yamada;Y.;Ueda;M.;et al.
• 通讯作者: et al.

Orthodontic Anchorage System Using a Locking Plate and Self-Drilling Screws

使用锁定板和自钻螺钉的正畸支抗系统

• 发表时间: 2006
• 期刊: Journal of Oral ＆ Maxillofacial Surgery 64
• 作者: Hibi;H.;Ueda;M.et al.
• 通讯作者: M.et al.

臍帯血からの間葉系幹細胞の分離と再生医療への応用

脐带血间充质干细胞的分离及其在再生医学中的应用

• 发表时间: 2004
• 期刊: 細胞 2
• 作者: Yamada;Y.;Ueda;M.;et al.;大屋 盛道 ほか;山田 陽一 ほか
• 通讯作者: 山田 陽一 ほか