Development technique of bone regeneration using Mesenchymal Stem Cell

利用间充质干细胞进行骨再生的开发技术

• 批准号: 11307048
• 负责人: UEDA Minoru
• 金额: $ 24.47万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11307048/
• 关键词: Cultured Bone; Tissue Engineering; Marrow Cell; Carrier; Bone Regeneration; 組織再生; 培養

Recent studies in the field of tissue engineering have developed and established the technique of cultured bone regeneration with mesenchymal stem cells (MSCs) derived from bone marrow. Most of characteristics of the regenerat ion have been clarified by these recent studies. Generally, it have described a new technology called tissue engin eering that involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaff olds and growth factors. In the present studies, we used MSCs as cell sources, ceramics and absorbable collagen as matrices, and rhBMP-2 and Platelet Rich Plasma as growth factors. In order to examine the effectiveness of cultur ed bone regeneration for clinical use, we investigated the following five things : 1) development of matrix, 2) ost eogenesis in vivo, 3) establishment of the method of larger scale of osteoblast culture, 4) the effectiveness of growth factors to improve the bone formation. 5) A possibility of clinical application in human. In regard to the matrix, we investigated the osteogenic potential of non resorbable HA and a new biodegradable β-tricalcium phosph ate (β-TCP), showing that β-TCP would be an ideal scaffolds for bone regeneration. In addition, we found that absorbable collagen would be an effective scaffold for rhBMP-2 in the sinus as well as autogenous bone. However, m ost studies regarding bone formation have adopted two dimensional histomorphometric evaluations and molecular biol ogys, not three dimensional such as micro CT. We also successfully evaluated microstructure of trabecular bone in human clinical cases. In some years from now, we are going to perform studies to improve the utility of the graft for the periodontal or alveolar defect for clinical use of technique of the cultured bone regeneration using MSCs with plasticity.

在组织工程领域的最新研究已经开发并建立了培养的骨骼再生技术，该技术是用骨髓衍生的间充质干细胞（MSC）（MSC）。这些最近的研究已经阐明了再生离子的大多数特征。通常，它描述了一种称为组织工程的新技术，该技术涉及由孤立细胞，生物相容性的SCAF型和生长因子形成的构造的新组织的形态发生。在本研究中，我们将MSC用作细胞来源，陶瓷和可吸收胶原蛋白作为物质，RHBMP-2和富含血小板的血浆作为生长因子。为了检查培养基ED骨再生对临床使用的有效性，我们研究了以下五件事：1）矩阵的发展，2）体内OST生态发生，3）建立大规模成骨细胞培养的方法，4）4）生长因子的有效性改善骨骼的形成。 5）在人类中临床应用的可能性。关于矩阵，我们研究了不可吸收的HA和新的可生物降解的β-三磷磷（β-TCP）的成骨潜力，表明β-TCP将是骨再生的理想支架。此外，我们发现可吸收的胶原蛋白将是窦和自动骨中RHBMP-2的有效支架。然而，关于骨形成的M OST研究采用了二维组织形态计量评估和分子生物OGY，而不是三维的三维（例如Micro CT）。我们还成功评估了小梁骨在人类临床病例中的微观结构。从现在开始的几年中，我们将进行研究，以改善移植物的牙周或肺泡缺陷的效用，以使用具有可塑性的MSC进行培养的骨再生技术的临床使用。

项目成果

Minoru Ueda: "Tissue Engineering in Bone and Cartilage"Transplant Today. 14.5. 612-618 (2001)

Minoru Ueda：“骨和软骨的组织工程”今日移植。

Minoru Ueda: "Hard and soft tissue engineering: applications in implant surgery"Tissues Engineering for Therapeu tic. 191-202 (2001)

Minoru Ueda：“硬组织工程和软组织工程：在种植手术中的应用”用于治疗的组织工程。

上田 実: "人体再生"現代医学. 49.1. 19-23 (2001)

上田稔：《人体再生》现代医学49.1。

上田 実: "ティッシュエンジニアリングの最新動向-幹細胞を利用した骨再生"The Quintessence Year Book 2001. 10-14 (2001)

Minoru Ueda：“组织工程的最新趋势 - 使用干细胞进行骨再生”The Quintessence Year Book 2001. 10-14 (2001)

Masaki Honda: "Cartilage Formation by Cultured Chondrocytes in a New Scaffold Made of Poly(I,-I, actide-ε-caprolaotone) sponge"Journal of Oral & Maxillofacial Surgely. 58. 767-775 (2000)

Masaki Honda：“由聚（I，-I，actide-ε-己内酯）海绵制成的新支架中培养的软骨细胞的软骨形成”口腔颌面外科杂志 58. 767-775（2000）。