Study of cultured bone or cartilage regeneration using human mesenchymal stem cells

利用人间充质干细胞培养骨或软骨再生的研究

• 批准号: 12794015
• 负责人: UEDA Minoru
• 金额: $ 23.68万
• 依托单位: Nagoya Univeristy
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for University and Society Collaboration
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12794015/
• 关键词: Cultured Bone; Cultured cartilage; graft materials; Mechanical Stress; Mesenchymal Stem Cells; Culture on a large scale; Cultured periosteum; ティッシュエンジニアリング; 大量培養法; ヒト

Recently, it is obvious that mesenchymal stem cells (MSCs) differentiate various human tissues and these cells proliferation technology comes true. On the other hand, biodegradable matrix with differentiation and proliferation of the cells developed by rapid development of matrix engineering. On the basis of these technology, tissue engineering, which involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaffolds and growth factor, was the focus of attention. By using this method, we can regenerate a living tissue using patioents' self. These can cure the difficult treatment of chronic bone and joint disease. At first, we pay attention to develop graft materials as a matrix and improve calcium phosphate. It called CPC and was compared to β-TCP. And in animal study with osteogenesis, it was clear that the materials had good ability of bone regeneration. Therefore we can expect application of the bone materials. And applying fibrin glue and platelet rich plasma, we can confirm that these material have ability of good bone regeneration. As for cell culture method, we can get acceleration of the cell function using stress, such as stretch stress, pressure stress and supersonic waves and apply bone regeneration. There were not big differences in function of chondrocytes, which differentiated from MSCs, using the same stress. And as for culture method on a large scale using a bioreactor, it was useful under using pressure stress. In the future, by applying these results, we would like to suggest cell providing method in regenerating bone and cartilage using MSCs and strategy of fostering new industry.

近年来，间充质干细胞（MSCs）分化各种人体组织的能力明显增强，并且这些细胞的增殖技术已成为现实。另一方面，在基质工程的快速发展的基础上，开发出具有细胞分化和增殖能力的生物可降解基质。在这些技术中，组织工程是人们关注的焦点，它涉及从分离的细胞、生物相容性支架和生长因子形成的结构中形成新组织，通过使用这种方法，我们可以利用患者自身再生活组织。治愈慢性骨关节疾病的治疗困难，首先我们关注开发作为基质的移植材料，并改进磷酸钙，并与β-TCP进行比较，并在动物研究中明确了这一点。材料具有良好的骨再生能力，因此我们可以期待骨材料的应用，并且应用纤维蛋白胶和富含血小板的血浆，我们可以证实这些材料具有良好的骨再生能力，而对于细胞培养方法，我们可以得到加速。利用压力来影响细胞功能，例如使用相同的应力，拉伸应力、压力应力和超声波以及应用骨再生，与MSC分化的软骨细胞的功能没有大的差异，并且对于使用生物反应器的大规模培养方法，其是有用的。未来，通过应用这些结果，我们希望提出利用间充质干细胞再生骨骼和软骨的细胞提供方法以及培育新产业的战略。

项目成果

Minoru Ueda: "Tissue engineering in research in oral implant surgery"Artificial Organs. 25・3. 164-171 (2001)

Minoru Ueda：“口腔种植外科研究中的组织工程”Artificial Organs 164-171（2001）。

上田 実: "これからの歯科は幹細胞の時代"The Quintessence. 21・1. 47-54 (2002)

Minoru Ueda：“牙科的未来是干细胞的时代”The Quintessence 21・1（2002）。

Minoru Ueda: "The potential of Regenerative medicine changing the dental therapy in 21 century"Journal of The Association for Tokyo Dental Doctor. 49-2. 47-59 (2001)

Minoru Ueda：“再生医学改变 21 世纪牙科治疗的潜力”东京牙科医师协会杂志。

Megmi Matsuno: "In vitoro Analysis Distraction osteogenesis"The Journal of Craniofacial Surgery. 11-4. 303-307 (2000)

Megmi Matsuno：“体外分析牵引成骨”颅面外科杂志。

Yoichi Yamada: "Injectable Bone"Clinical Calcium. 12-2. 50-54 (2002)

山田洋一：“可注射骨”临床钙。