Bone Cells Analysis

骨细胞分析

基本信息

批准号：
10183172
负责人：
PAOLA DIVIETI PAJEVIC
金额：
$ 24.14万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-06-19 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10183172
关键词：
3-Dimensional Animal Model Architecture Biological Assay Biological Markers Biology Blood Boston Cartilage Cell Line Cell Separation Cells Clinical Collaborations Communities Consultations Cost efficiency Cultured Cells Cyclic AMP DNA Sequencing Facility Data Educational workshop Endocrine Fees Fostering Funding Genes Genetic Genetically Modified Animals Goals Human In Situ Hybridization In Vitro Informatics Leadership Libraries Measurement Mentors Methodology Methods Mission Morphology Mus Pediatric Hospitals Performance Population Process Property Protocols documentation RNA RNA analysis Reagent Recovery Research Research Design Research Personnel Resources Route Sampling Scientific Advances and Accomplishments Series Services Signal Pathway Signal Transduction Signal Transduction Pathway Signaling Molecule Specimen Structure System Techniques Technology Tissues Total Hip Replacement Training Universities Urine Work bone bone cell cost cost effective design established cell line experimental study genetic analysis genetic manipulation human subject in vivo innovation instrumentation interest investigator training molecular dynamics novel operation pre-clinical programs quality assurance scaffold single-cell RNA sequencing skeletal skeletal tissue tissue/cell preparation tool trafficking transcriptome sequencing transcriptomics

项目摘要

The proposed Bone Cells Analysis Core (BCAC) will enable participating investigators to pursue new lines of research in skeletal biology by using state-of-the-art technologies for bone cell isolation, functional assessment, and RNA sequencing. The proposed Core will bring together the expertise of its leadership (Director Dr. Pajevic Divieti and the Associate Directors Drs. Gardella, Gerstenfeld and Warman) and powerful new research approaches and assay systems to make for a synergistic Core Resource that will greatly enrich and catalyze the scientific advances of participating investigators. The BCAC will provide Center investigators (CIs) with 1) Preclinical and clinical bone cells and genetic tools, 2) Cell Signaling and Biomarker Assay services, and 3) RNA sequencing services. The Bone cell Core (Drs Divieti Pajevic and Gerstenfeld, Boston University), will provide mouse- derived bone cells and cells lines from both wild type or genetically modified animals. The Core will also provide, in collaboration with Dr. Gerstenfeld (Associate Director), highly annotated human specimens from total hip replacements. The BCAC will offer a wide array of in-house and commercially available signaling assays under the Signaling Assay service (Dr. Gardella, Associate Director, Endocrine Unit, MGH). The Core will help with the study design and will train investigators in all the aspects of these techniques. The RNA sequencing core (Dr. Warman, Associate Director, Boston Children’s Hospital) will provide services to help CIs and their trainees design, perform, and analyze bulk and single cell RNA sequencing experiments that involve skeletal cells and tissues. The Core will provide help with designing bulk and single cell RNA sequencing experiments and with assisting with tissue and cell preparation for RNA recovery. The core will provide micro-pulverizers and optimized methods for recovering RNA from bone and cartilage for bulk RNA sequencing and living cells for single cell RNA sequencing. The Core will generate sequencing libraries using kits designed for very low amounts of starting RNA. The Core will assist in the informatics analysis of the sequencing data. Each service provided by the Core will be rigorously controlled for quality performance and reliability. The Core will conduct hands-on training sessions by which investigators can be mentored and taught how to perform assays, cell manipulations on their own and RNA sequencing. It will also establish a seminar series in which new methodologies, approaches, and related scientific progress will be presented and discussed. !

支撑骨细胞分析核心（BCAC）将使参与的研究人员能够追求使用骨细胞的最新技术，骨骼生物学的新研究线隔离，功能评估和RNA测序。专业知识OTS领导 Gerstenfeld和Warman）以及强大的新研究方法和测定系统一种协同的核心资源，它将充实和催化科学进步参与调查人员。 BCAC将为中心研究人员（CIS）提供1）临床前和临床骨细胞以及遗传工具，2）细胞信号传导和生物标志物测定服务，以及3）RNA测序服务。骨细胞核心（Drs Divieti Pajevic和Gerstenfeld，波士顿大学）将提供小鼠 - 来自野生型或转基因动物的骨细胞和细胞还与Gerstenfeld博士（副主任）合作提供了高度注释的人总髋关节替代品的标本。信号屁股测定服务下的市售信号传导测定（Gardella博士， MGH内分泌副主任。研究人员在技术的所有方面。波士顿儿童医院副主任）将提供服务以帮助CI及其培训设计，执行和分析涉及的大量和单细胞RNA测序实验骨骼细胞和组织都可以帮助设计散装和单细胞RNA 测序实验并有助于将什一税和细胞制备进行RNA恢复核心将延长微塑料和优化的方法，以从骨头和用于单细胞RNA测序的大块RNA测序和活细胞的软骨使用针对非常低量的启动RNA设计的套件来生成测序库将协助对测序数据的信息分析。核心提供的每种服务将受到严格控制的质量性能和可靠性。指导并教导如何进行测定，单元进行操作和RNA序列。它还将建立一个研讨会系列，其中新方法，方法和相关的方法科学进步将被主持并讨论。呢