Development of a High Precision Three-Dimensional Modeling System for Biological Samples

生物样品高精度三维建模系统的开发

基本信息

批准号：
14205036
负责人：
HIGUCHI Toshiro
金额：
$ 27.96万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Using a Micro-Slicer Image Processing System, which consists of a rotary blade and automatic sample feeding mechanism, biological samples such as food and internal organs have been observed. It became possible to observe the distribution of ice crystals formed in frozen foods and biological materials with this system. To observe the distribution of ice crystals a fluorescence stain was used and an optimal wavelength of light was selected. The processes and results were presented in prepublication papers shown below.・Quantitative Analysis of Ice Crystals Formed in Frozen Food (Japanese Society for Cryobiology and Cryotechnology)・Three-Dimensional Measurement of Ice Crystals in Frozen Dilute Solutions (Japan Society of Refrigerating and Air conditioning Engineers)・None-destructive Measurement of Ice Crystals in Frozen Gelatin Gel-Estimation of Ice Crystal Structure by Utilizing Dielectric Properties-(Japan Society of Refrigerating and Air conditioning Engineers)An award was given for the paper of "None-destructive Measurement of Ice Crystals in Frozen Gelatin Gel" by Japan Society of Refrigerating and Air conditioning Engineers.A new type of Micro-sheer image processing system was proposed. A linear motor moves a slicing blade. This system is not equipped with a CCD camera and lens, but it uses an image scanner for image acquisition. In this system, a sample is sliced and the images of sliced sample are acquired automatically and continuously. Compared with the conventional systems, the images of the sliced sample are acquired with a high degree of accuracy because the sample is illuminated uniformly. Furthermore, the system is more compact because translatory movement of a slicing blade does not need as much space as the rotary movement. After the acquisition of imge data of each sliced sample, a three dimensional image, showing the internal information of the biological sample, can be reconstructed on a computer.

使用旋转叶片和自动样品喂养机制组成的微型图像处理系统，已经观察到了食物和内部器官等生物样品。可以观察到使用该系统在冷冻食品和生物材料中形成的冰晶的分布。为了观察冰晶的分布，使用了荧光染色，并选择了最佳的光波长。在下面显示的准备论文中呈现了这些过程和结果。・对冷冻食品中形成的冰晶体（日本冷冻生物学和冷冻技术学会）・对冰晶溶液中冰晶体的三维测量（日本的稀释和空调工程师的日本社会）的三维测量通过利用介电特性（日本冷藏和空调工程师协会）通过日本冷藏和空调工程师协会的“无损于冰晶的冰晶测量”的论文颁发了结构。线性电机移动切片刀片。该系统不配备CCD摄像头和镜头，但它使用图像扫描仪进行图像采集。在此系统中，切成样品，切成薄片样品的图像自动获取。与常规系统相比，切成薄片样品的图像以高度准确性获取，因为样品均匀地照亮。此外，该系统更紧凑，因为切片刀片的译动运动不需要旋转运动的空间。在获取每个切片样本的IMGE数据之后，可以在计算机上重建一个三维图像，显示生物样品的内部信息。