Functional recovery after nerve transplantation to optic nerve

视神经神经移植术后功能恢复

基本信息

批准号：
13671837
负责人：
SASAKI Hitoshi
金额：
$ 2.62万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Reconstruction of visual pathway can be achieved by the peripheral nerve transplantation to the cut end of the optic nerve. It has been reported that the restored visual pathway can conduct some visual information such as light-dark. In this research we have tried to evaluate motion information can be processed by the restored visual system, using prepulse inhibition with a motion stimulus as a prepulse. To obtain this result, animal's visual field should be fixed. Thus we have tried to record prepulse inhibition under alpha-chloralose anesthesia. However, clear prepulse effects could not be obtained in this condition in normal control rats. Next, we have tried to evaluate detection of motion stimulus using shuttle-box avoidance task. A motion of sine-wave gratings was used as a conditioned stimulus. Unconditioned stimulus was a shock from the floor of the box. Clear learning curves have been obtained with a motion speed of 40, 20, 1.5,0.5 deg/s, showing avoidance rate of 55-70% at the 5th day of training. Nine transplanted rats have been tested using prepulse inhibition with a white LED as a prepulse. One rat showed a tendency of inhibition effect. This rat was tested in the shuttle-box using a motion stimulus of 20 deg/s. Avoidance rate increased as progress of training sessions. After the test with 20 deg/s, same training has been done with a motion speed of 40 deg/s. However, no learning effect was observed. Another transplanted rat, showing no prepulse effect to LED prepulse was tested as a control for shuttle-box task. No improvement of avoidance rate has been observed. These results suggest that some transplanted rat might be able to detect motion

视觉途径的重建可以通过外周神经移植到视神经的切割端来实现。据报道，恢复的视觉途径可以传达一些视觉信息，例如浅黑色。在这项研究中，我们试图使用以运动刺激作为预硫的预硫抑制，可以通过恢复的视觉系统来处理运动信息。为了获得此结果，应固定动物的视野。因此，我们试图记录在α-氯洛糖麻醉下的预硫抑制作用。但是，在这种情况下，在正常对照大鼠中无法获得明显的预硫效应。接下来，我们尝试使用避开式避免任务来评估运动刺激的检测。正弦光栅的运动用作条件刺激。无条件的刺激是盒子地板的冲击。明确的学习曲线以40、20、1.5,0.5度/s的运动速度获得，在训练的第五天显示回避率为55-70％。使用白色LED作为预硫的预硫抑制，已经对9只移植大鼠进行了测试。一只大鼠显示出抑制作用的趋势。使用20度/s的运动刺激在穿梭盒中测试了这只大鼠。随着培训课程的进展，回避率提高了。测试20度/s后，已经以40度/s的运动速度进行了相同的训练。但是，未观察到学习效果。另一个移植的大鼠，显示对LED预硫的预硫效应没有被测试作为穿梭盒任务的控制。没有观察到回避率的提高。这些结果表明，一些移植的大鼠可能能够检测运动