Establishment of evidence based antibacterial chemotherapy

建立循证抗菌化疗

基本信息

批准号：
13671651
负责人：
OKADA Hiroshi
金额：
$ 2.3万
依托单位：
Teikyo University (2003-2004)Kobe University (2001-2002)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2004
项目状态：
已结题

项目摘要

A new automated flow cytometry based urine bacteria analyzer, UBA, was developed. We assessed the UBA for linearity of measurement, reproducibility of results, carryover rate, and correlation of the measured results with those determined by urine culture. We also evaluated for its ability to screen urine samples for significant bacteriuria.The UBA showed excellent linearity and minor carryover rate. Results from the UBA were highly reproducible, and in between-run precision assays, the coefficients of variation for UBA results were smaller than those by the urine culture.Two hundred and seventy-three urine specimens from patients attending outpatient clinic of university based hospital were examined. The results from the UBA were compared with those from urine culture.The UBA detected significant bacteria with sensitivity 96.6%, specificity 79.9%, positive predictive value 57.0%, negative predictive value 98.8%, false positive rate 15.8%, false negative rate 0.7%, and accuracy 83.5%. These results were comparable to or better than those obtained with previously reported screening procedures.The UBA can perform accurate enumeration of bacterial cells automatically in 90 seconds, and can be used for the screening of significant bacteriuria. Moreover, the UBA can be used to develop a new device to determine the susceptibilities of bacteria in urinary tract infections.

开发了一种新型基于自动化流式细胞术的尿液细菌分析仪 UBA。我们评估了 UBA 的测量线性、结果再现性、残留率以及测量结果与尿培养测定结果的相关性。我们还评估了其筛查尿液样本中显着菌尿的能力。UBA 显示出出色的线性度和较小的残留率。 UBA 结果具有高度可重复性，并且在运行间精密测定中，UBA 结果的变异系数小于尿培养结果。对来自大学附属医院门诊患者的 273 份尿液样本进行了分析检查了。将UBA结果与尿培养结果进行比较，UBA检出显着细菌，敏感性96.6%，特异性79.9%，阳性预测值57.0%，阴性预测值98.8%，假阳性率15.8%，假阴性率0.7% ，准确率83.5%。这些结果与之前报道的筛查程序获得的结果相当或更好。UBA可以在90秒内自动对细菌细胞进行准确计数，可用于筛查显着菌尿。此外，UBA 可用于开发一种新装置来确定尿路感染细菌的敏感性。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Enumeration of bacterial cell numbers and detection of significant bacteriuria by a new flow cytometry-based device.

通过新型流式细胞术设备计数细菌细胞数并检测显着菌尿。

DOI：
发表时间：
期刊：
Am.J.Clin.Pathol. (Submitted for publication)
影响因子：
0
作者：
Hiroshi Okada;Toshiro Shirakawa;Katsumi Shigemura;Kazushi Tanaka;Soichi Arakawa;Akinobu Gotoh;Yukio Hamaguchi;Sadao Kamidono
通讯作者：
Sadao Kamidono

Enumeration of bacterial cell numbers and detection of significant bacteriuria by new flow cytometry-based device.

通过基于流式细胞术的新型装置对细菌细胞数进行计数并检测显着菌尿。

DOI：
发表时间：
期刊：
Am.J.Clin.Pathol. (Submitted for publication)
影响因子：
0
作者：
Hiroshi Okada;Toshiro Shirakawa;Katsumi Shigemura;Kazushi Tanaka;Soichi Arakawa;Akinobu Gotoh;Yukio Hamaguchi;Sadao Kamidono
通讯作者：
Sadao Kamidono

Hiroshi Okada: "Evaluation of the Sysmex UF-50"Am.J.Clin Pathol.. 115. 605-610 (2001)

Hiroshi Okada：“Sysmex UF-50 的评估”Am.J.Clin Pathol.. 115. 605-610 (2001)