The influence of chromosome polymorphisms of Ranunculus silerifolius (Ranunculaceae) to genetic diversity of populations

毛茛科毛茛染色体多态性对群体遗传多样性的影响

基本信息

批准号：
14596006
负责人：
OKADA Hiroshi
金额：
$ 2.11万
依托单位：
Osaka City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

Gene flow between partially reproductively isolated Otaru and Matsuyama cytotypes of Ranunculus silerifolius was revealed using allozyme polymorphisms. Otaru and Matsuyama cytotypes in the parapatric population MIX were distinguishable critically by the different alleles of the Mdh-3 allozyme locus, and the hybrids between both cytotypes shared both alleles of both cytotypes. The distribution pattern of the alleles of the Mdh-3 locus revealed that the gene flow did not occur between cytotypes within MIX. The value of gene flow between the two cytotypes within the parapatric population MIX (i.e.0.503) was smaller than those between allopatric populations of the same cytotype (i.e.2.550 or 1.700). The genetic distance between cytotypes within the parapatric population MIX was larger than those between the far distant populations of the same cytotypes. Dendrograms based on the Nei's genetic distance using the variations of allozyme markers showed that Otaru and Matsuyama cytotypes formed … More clusters separately.Genetic variations of the facultatively autogamous species Ranunculus silerifolius, which showed intra-specific chromosomal polymorphisms (Karatsu-, Matsuyama-, Mugi, and Otaru-cytotype), were investigated in 95 populations using allozymic analysis. Sixteen loci of nine enzyme systems were resolved. Three loci were monomorphic for all populations. On average, 26% of the loci were polymorphic ; the 16 loci resolved gave a total of 49 alleles with a mean number of 1.29 alleles per locus. Overall levels of genetic variation were low as with other facultatively autogamous species. The gene differentiation coefficient among populations, G_<ST> was high (0.579), which probably reflected the inbreeding in R.silerifolius. The absence of a correlation between genetic distance and geographical distance may have resulted from the same effect. The average of genetic distance between populations was large (D=0.129), indicating a high level of differentiation and low level of gene flow (Nm=0.171) among the populations, while that between cytotypes was very small (D=0.008), possibly due to the relatively short period of fine for genetic diversification since the occurrence of the cytotypes. Less

基因在使用同化的杂种型毛y山的细胞型中，基因流动在孢子种群中混合物。在隔型种群中的细胞型之间的基因流量（即0.503）是同种异体种群（即2.550或1.700）之间的较小的基因。 G同种酶标记物的变化显示出otaru和Matsuyama细胞型……对LY自动物种ranunculus silerifolius的分离簇显示出95个流行的95个流行分析。基因座是多态性的； 16个基因座的平均数量为49个，每个基因座的平均数量为1.29个。在R.Silererifology中，地理距离之间的相关性可能很大（D = 0.129），表明细胞型很小（D = 0.008）。细胞型降低