Analysis of transient expression of estrogen receptor in the newborn rat brain

新生大鼠脑内雌激素受体瞬时表达分析

基本信息

批准号：
13640667
负责人：
HAYASHI Shinji
金额：
$ 2.3万
依托单位：
YOKOHAMA CITY UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

We have found that estrogen receptor (ER) alpha is expressed transiently in the facial nucleus and in the primary auditory cortex of new born rats. However, biological meaning of this expression is still left unexplained. Using TUNEL method, we failed to detect any apoptotic signals in the facial nucleus of fetal to newborn rats between 16 days after conception (FE16) and 11days after birth (PN11). Thus, it is suggested that the cells expressing ER alpha are not those die early in the life. On the other hand, the birth date of the ER alpha positive cells was estimated by giving BrdU to pregnant mothers followed by immunohistochemical detection of ER signals. Colocalization of the BrdU and ER alpha signals was only detected in those samples given BrdU at 11 and 12 days after conception (FE11-12). Thus, motoneurons in the facial nucleus is suspected to attain final cell division around FE11-12 and only a part of them express ER alpha. Since ER signals disappear after PN14, our hypothesis that only immature motoneurons contain ER signals is supported. On the other hand, we examined in the primary auditory cortex of the newborn rats if ER signals were co-expressed with any neuropeptides, such as neuropeptides Y (NPY), gleaning, substance P, cholecystokinine 8 (CCK8), and vasoactive intestinal peptide (VIP) by using double immunohistochemistry method. So far we failed to detect any distinct Colocalization of ER alpha signals with any of these peptides. However, since we detected ER alpha signals in extranuclear loci by electron microscopy in the hippocampal pyramidal neurons, there is a possibility of extra-nuclear action of estradiol through estrogen receptor.

我们发现，雌激素受体（ER）α在面部核和新生大鼠的主要听觉皮层中瞬时表达。但是，这种表达的生物学含义仍然无法解释。使用TUNEL方法，我们未能检测到受孕后16天（FE16）和出生后11天（PN11）之间16天之间胎儿对新生大鼠面部核中的任何凋亡信号（PN11）。因此，建议表达ERα的细胞不是生命早期死亡的细胞。另一方面，通过将BRDU给怀孕的母亲，然后对ER信号进行免疫组织化学检测来估计ERα阳性细胞的出生日期。仅在受孕后11和12天给出的BRDU的样品中检测到BRDU和ERα信号的共定位（FE11-12）。因此，怀疑面部核中的运动神经元在Fe11-12左右获得最终细胞分裂，并且只有一部分表达ERα。由于PN14之后的ER信号消失，因此我们的假说仅支持未成熟的运动神经元包含ER信号。另一方面，我们在新生大鼠的主要听觉皮层中检查了ER信号是否与任何神经肽（例如Neuropeptides Y（NPY），Glaning，Gleaning，septs P，CCK8）和血管活性二肽（VIP（VIP）（VIP）使用双重肽（VIP）的任何神经肽肽共表达。到目前为止，我们还没有检测到ERα信号与任何这些肽的任何明显的共定位。但是，由于我们通过电子显微镜在海马锥体神经元中检测到核外基因座中的ERα信号，因此雌二醇通过雌激素受体有核外作用。