ESTROGEN RECEPTOR DETECTED IN THE FACIAL NUCLEUS OF NEWBORN RATS.

在新生大鼠的面核中检测到雌激素受体。

• 批准号: 05804050
• 负责人: HAYASHI Shinji
• 金额: $ 1.15万
• 依托单位: TOKYO METROPOLITAN INSTITUTE FOR NEUROSCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05804050/
• 关键词: Estrogen Receptor; Motoneuron; Development; Immunohistochemistry; Gene Expression; Facial Nucleus; Auditory Cortex; Rat; 運動ニユウロン; 一過性出現; 新生仔

Distribution and ontogeny of estrogen receptor (ER) -containing neurons in the newborn rat brain was examined. Neurons containing ER were classified into two types, i.e.,(1) Adult-type : the ER neurons appear in the same regions in the newborn as those in the adult ;(2) Newborn-type : the ER appears transiently only during the fetal to newborn period. The adult-type was observed in the preoptic nucleus, in the bed nucleus of the stria terminalis, in the hypothalamic arcuate, ventromedial and periventricular nuclei, in the midbrain central gray, in the lateral habenular nucleus, and medial and cortical nuclei of the amygdala. On the other hand, the newborn-type appears only in the ventromedial subnucleus of the facial nucleus and the layr 5 of the primary auditory cortex. Estrogen administration decreases ER immunoreactivity in the both types of ER.ER mRNA expression was detected by in situ hybridization in both cases. However, the area where former group was found contains aromatase immunoreactivity, which indicates androgen is converted to estradiol, while that for the latter does not. Sex difference was found only the former group. Thus, ER directly related to sex differentiation of the brain was suggested as the former (adult-type), while the latter (newborn-type) might be related to stimulating effects of estrogen in the developing brain. By retrograde track-tracing, double immunohistochemical staining of ER and choline acetyltransferase, and electron microscopic immunocytochemistry, the cells which contain ER transiently in the newborn life were suggested as immature motoneurons.

检查了新生大鼠脑中雌激素受体（ER）神经元的分布和个体发育。包含ER的神经元分为两种类型，即（1）成人类型：ER神经元与成年人中的新生儿相同区域出现；（2）新生儿型：ER仅在胎儿到新生儿时期出现暂时性。在末端末端的床核中观察到成年型，在下丘脑弧形，腹膜和脑室核中，在中脑中央灰色中的中脑灰色，外侧Habenular nubleus核，以及伴有amygdala的内侧和皮质核。另一方面，新生儿型仅出现在面部核的腹侧核和主要听觉皮层的Layr 5中。两种情况下，通过原位杂交检测到两种类型的ER.ER mRNA表达中雌激素的ER免疫反应性降低。但是，发现以前的组含有芳香酶免疫反应性，这表明雄激素转化为雌二醇，而后者则没有。性别差异仅发现了前群体。因此，ER与大脑的性别分化直接相关，是前者（成人类型），而后者（新生儿型）可能与雌激素在发育中的大脑中的刺激作用有关。通过逆行轨道追踪，ER和胆碱乙酰转移酶的双重免疫组织化学染色以及电子显微镜免疫细胞化学，在新生儿寿命中含有ER的细胞被认为是未成熟的运动神经元。

项目成果

Hayashi,S.: "Immunocytochemical detection of estrogen receptor in the facial nucleus of the newborn rat by three antibodies with distinct epitopes." Hormones and Behavior. 28. 530-536 (1994)

Hayashi,S.：“通过具有不同表位的三种抗体对新生大鼠面核中的雌激素受体进行免疫细胞化学检测。”

Okamura,H.,Yokosuka,M.and Hayashi,S.: "Induction of substance P-immunoreacitvity by estrogen in neurons containing estrogen receptor in the anteroventral perivetricular nucleus of female but not male rats." J.Neuroendocrinology,. 6. 609-615 (1994)

Okamura,H.、Yokosuka,M. 和 Hayashi,S.：“雌激素在雌性而非雄性大鼠前腹侧周核中含有雌激素受体的神经元中诱导 P 物质免疫反应性。”

Kikuta,T.,Yamamoto,k.,Namiki,H.and Hayashi,S.: "Immunocytochemical localization of estrogen receptor in various anterior pituitary hormone cells of adult male and female rats." Acta Histochem.Cytochem.26. 609-614 (1993)

Kikuta,T.、Yamamoto,k.、Namiki,H. 和 Hayashi,S.：“成年雄性和雌性大鼠各种垂体前叶激素细胞中雌激素受体的免疫细胞化学定位。”

Orikasa, C., Okamura, H.and Hayashi, S.: "Estrogen found in the facial nucleus of the newborn rat is suppressed by exogneous estrogen : Immuno-and in situ hybridization histochemical studies." Dev.Brain Res.82. 9-17 (1994)

Orikasa, C.、Okamura, H. 和 Hayashi, S.：“新生大鼠面核中发现的雌激素被外源雌激素抑制：免疫和原位杂交组织化学研究。”

Yokosuka, M., Okamura, H.and Hayashi, S.: "Transient expression of estrogen receptor-immunoreactivity (ER-IR) in the layr V of the developing rat cerebral cortex." Dev.Brain Res.84. 99-108 (1995)

Yokosuka, M.、Okamura, H. 和 Hayashi, S.：“发育中的大鼠大脑皮层第 V 层中雌激素受体免疫反应性 (ER-IR) 的瞬时表达。”