Steroid Hormone Receptor-Containing Neurons and Differentiation of Neuroendocrine Functions

类固醇激素受体神经元与神经内分泌功能的分化

• 批准号: 09640799
• 负责人: HAYASHI Shinji
• 金额: $ 1.98万
• 依托单位: TOKYO METROPOLITAN INSTITUTE FOR NEUROSCIENCE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09640799/
• 关键词: Estrogen Receptor; Androgen Receptor; Aromatase; Immunohistochemistry; RT-PCR; Gene expression; Hypothalamus; Hippocampus; エストロゲン受容体

Role of neurons that contain steroid hormone receptors, such as estrogen or androgen receptors in the development and differentiation of the brain was analyzed. By using immunohistochemistry (IHC), in situ hybridization histochemistry (ISHH) and reverse transcriptase-polymerase chain reaction (RT-PCR), we have already disclosed that estrogen receptor alpha (ERalpha) is expressed in the preoptic region, the hypothalamus and the amygdala in the rat brain. Moreover, we have disclosed ERalpha is also detected in the hylar region of the hippocampus both by IHC and ISHH.They were located sporadically in the polymorphic neurons of the hylus and granular cells of the cornus. No distinct sex difference was detected. In addition, androgen receptor immunoreactivity was detected selectively in the ventral premammillary nucleus that is located in the posterior region of the hypothalamus. The androgen receptor immunoreactivity in this nucleus increased by administration of androgen. Since no aromatase that converts testosterone into estradiol was detected in this nucleus, direct action of androgen on the neurons of this nucleus was suggested. On the other hand, a new kind of estrogen receptor that was found recently in the rat prostate and named as ERbeta is also detected in the brain. We also confirmed the expression of ERbeta in the rat brain by IHC and ISHH.

分析了含有类固醇激素受体（例如雌激素或雄激素受体）的神经元在大脑发育和分化中的作用。通过免疫组织化学（IHC）、原位杂交组织化学（ISHH）和逆转录聚合酶链反应（RT-PCR），我们已经揭示雌激素受体α（ERα）在视前区、下丘脑和杏仁核中表达在老鼠的大脑中。此外，我们还发现，通过IHC和ISHH，在海马的海拉区也检测到了ERα。它们零星地位于海马的多态性神经元和眼角的颗粒细胞中。没有检测到明显的性别差异。此外，在位于下丘脑后部区域的腹侧前乳头核中选择性地检测到雄激素受体免疫反应性。通过给予雄激素，该细胞核中的雄激素受体免疫反应性增加。由于在该细胞核中没有检测到将睾酮转化为雌二醇的芳香酶，因此表明雄激素对该细胞核的神经元有直接作用。另一方面，最近在大鼠前列腺中发现的一种新型雌激素受体，命名为ERbeta，也在大脑中检测到。我们还通过 IHC 和 ISHH 证实了大鼠脑中 ERbeta 的表达。

项目成果

Hayashi,S.,et al.: "Neural Control of Reproduction: Physiology and Behavior" Maeda,K.and Tsukamura,H.(eds.)日本学会出版センター、東京, 135-152 (1997)

Hayashi, S. 等人：“生殖的神经控制：生理学和行为”Maeda, K. 和 Tsukamura, H.（编辑）日本协会出版中心，东京，135-152（1997）

Yang, Y., Ozawa, H., Lu, H., Yuri, K., Hayashi, S., Nihonyanagi, K.and Kawata, M.: "Immunocytochemical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion, with special reference to estroger and its receptor." Br

Yang, Y.、Ozawa, H.、Lu, H.、Yuri, K.、Hayashi, S.、Nihonyanagi, K. 和 Kawata, M.：“大鼠降钙素基因相关肽性别差异的免疫细胞化学分析

Yang,Y.,et al.: "Immunocytochenmical analysis of sex differences in calcitonin gene-related peptide in the rat dorsal root ganglion,with special reference to estrogen and its receptor." Brain Res. 791. 35-42 (1998)

Yang，Y.，et al.：“大鼠背根神经节降钙素基因相关肽性别差异的免疫细胞化学分析，特别是雌激素及其受体。”

Alves, S., Weiland, N.G., Hayashi, S.and McEwen, B.S.: "Immunocytochemical localization of nuclear estrogen receptors and progesterone receptors within the rat dorsal raphe nucleus" J Comp Neurol. 391. 322-334 (1998)

Alves, S.、Weiland, N.G.、Hayashi, S. 和 McEwen, B.S.：“大鼠中缝核内核雌激素受体和孕激素受体的免疫细胞化学定位”J Comp Neurol。

Hayashi,S.: "Advances in Comparative Endocrinology" Kawashima,S.and Kikuyama,eds.S.Monduzzi Editore S.p.A.Bologna,Italy, 1823-1828 (1997)

Hayashi,S.：“比较内分泌学的进展”Kawashima,S. 和 Kikuyama,eds.S.Monduzzi Editore S.p.A.博洛尼亚，意大利，1823-1828 年（1997 年）