Sex Hormone - Mediated Differentiation of Neuroendocrine System

性激素 - 介导神经内分泌系统的分化

基本信息

批准号：
09044245
负责人：
HAYASHI Shinji
金额：
$ 2.94万
依托单位：
Tokyo Metropolitan Institute for Neuroscience
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

项目摘要

Involvement of sex steroid hormones such as estrogen and androgen in development and determination of neuroendocrine functions has been well established . In this project , we examined distribution of receptor signals for these steroid hormones in the central and peripheral nervous systems in the developing rat brain by immunohistochemistry ( IHC ) and by in situ hybridization histochemistry ( ISHH ) . The following points were revealed by a series of experiments : (1) The immunoreactivities ( ir ) for estrogen receptor a (Era ) were detected in those regions that are related to reproductive functions , such as the preoptic area , the hypothalamic arcuate nucleus , ventrolateral part of the hypothalamic ventromedial nucleus , and the medial amygdala nucleus ; (2) There was clear sex difference in the expression of Era , stronger in the female than the male , in the specific regions wherearomatase - ir was detected . This observation supports the concept that androgen of the testicular origin is converted into estrogen , and then suppresses Era expression ( down - regulation ) ; (3) Era signals were also detected in the regions such as in the cerebral cortex , the hippocampus , the midbrain central gray , although they were rather scattered but clearly . The Era signals in the hippocampus and the midbrain central gray were in the interneurons ; (4) We also detected Era - ir signals in the dorsal root ganglia where they were colocalized with the calcitonin generelated peptide ( CGRP ) . The finding suggests estrogen is involved in regulation of CGRP expression .

雌激素和雄激素等性类固醇激素参与神经内分泌功能的发育和测定已经得到充分证实。在这个项目中，我们通过免疫组织化学 (IHC) 和原位杂交组织化学 (ISHH) 检查了发育中的大鼠大脑中枢和周围神经系统中这些类固醇激素受体信号的分布。通过一系列实验揭示了以下几点： ( 1 )在与生殖功能相关的视前区、下丘脑弓状核、腹外侧区等区域检测到了雌激素受体a (Era )的免疫反应性( ir ) 。下丘脑腹内侧核和内侧杏仁核的一部分； (2)在检测到芳香化酶-ir的特定区域中，Era的表达存在明显的性别差异，女性表达强于男性。这一观察结果支持了睾丸来源的雄激素转化为雌激素，然后抑制Era表达（下调）的观点。 (3)大脑皮层、海马、中脑中央灰等区域也检测到Era信号，虽然比较分散，但清晰。海马和中脑中央灰质的Era信号位于中间神经元； (4)我们还在背根神经节中检测到了Era-ir信号，它们与降钙素基因相关肽(CGRP)共定位。这一发现表明雌激素参与了 CGRP 表达的调节。