The relationship between activation ofneurotransmitters by the mastication, learning, and memory : investigation of the mechanism by chronobiological method

咀嚼、学习和记忆激活神经递质之间的关系：通过时间生物学方法研究其机制

基本信息

批准号：
12672005
负责人：
YOSHIHARA Toshihiro
金额：
$ 1.73万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672005/
关键词：
learning memory restricted daily feeding corticosterone

项目摘要

The purpose of the present study was to develop a new system for estimating the level of learning and memory of rats by chronobiological method, and to examine whether the mastication affects learning and memory.It is well known that the temporal profile of hypothalamus-pituitary-adrenocortical axis in rats are modified by restricted daily feeding (RF), in which food supply is restricted to a fixed time of day. Under RF, a corticosterone peak appears in 8 to 10 days, from 2 hours before the daily meal supply (anticipatory peak), and which is detectable for more than several weeks even after the termination of RF. The anticipatory corticosterone peak is regarded as an expression of endogenous circadian rhythm associated with the feeding schedule. In this study, this phenomenon was used for estimating the level of learning and memory of rats. The following results was obtained.(1) The anticipatory corticosterone peak did not appear under restricted daily parenteral feeding or under restricted daily nutrition via jugular vein. These results suggested that supplement nutrition was needed for the development of the anticipatory corticosterone peak.(2) Restricted daily solid meal produced the anticipatory hormone peak in 8 days, while restricted daily liquid meal produced the peak in 12 days. The developed hormone peak was less prominent by liquid meal than by solid.(3) The anticipatory corticosterone peak developed by solid meal persisted throughout the period examined (3 weeks) after the termination of RF, while the peak developed by liquid meal disappeared after the first week.It is concluded that liquid meal attenuates the meal anticipation in the adrenocortical activity in rats under RF. These results suggested that the mastication affected the development and persistence of memory in rats.

本研究的目的是开发一种新的系统，通过时间生物学方法评估大鼠的学习和记忆水平，并检查咀嚼是否影响学习和记忆。众所周知，下丘脑-垂体-的时间分布限制每日进食（RF）可以改变大鼠的肾上腺皮质轴，其中食物供应仅限于一天中的固定时间。在 RF 治疗下，皮质酮峰值出现在 8 至 10 天内，从每日进餐前 2 小时开始（预期峰值），并且即使在 RF 终止后数周以上也可检测到。预期皮质酮峰值被认为是与喂养时间表相关的内源性昼夜节律的表达。在这项研究中，这种现象被用来评估老鼠的学习和记忆水平。得到如下结果：(1)限制每日肠外喂养或限制每日颈静脉营养时未出现预期皮质酮峰值。这些结果表明，预期皮质酮峰值的形成需要补充营养。(2)限制每日固体膳食在8天内产生预期激素峰值，而限制每日流质膳食在12天内产生预期激素峰值。流质膳食产生的激素峰不如固体膳食显着。(3)固体膳食产生的预期皮质酮峰在RF终止后的整个检查期间(3周)持续存在，而流质膳食产生的峰在RF终止后消失。第一周。得出的结论是，流质膳食减弱了射频下大鼠肾上腺皮质活动的进餐预期。这些结果表明咀嚼影响大鼠记忆的发展和持久。