The relationship between activation ofneurotransmitters by the mastication, learning, and memory : investigation of the mechanism by chronobiological method

咀嚼、学习和记忆激活神经递质之间的关系：通过时间生物学方法研究其机制

基本信息

批准号：
12672005
负责人：
YOSHIHARA Toshihiro
金额：
$ 1.73万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672005/
关键词：
learning memory restricted daily feeding corticosterone

项目摘要

The purpose of the present study was to develop a new system for estimating the level of learning and memory of rats by chronobiological method, and to examine whether the mastication affects learning and memory.It is well known that the temporal profile of hypothalamus-pituitary-adrenocortical axis in rats are modified by restricted daily feeding (RF), in which food supply is restricted to a fixed time of day. Under RF, a corticosterone peak appears in 8 to 10 days, from 2 hours before the daily meal supply (anticipatory peak), and which is detectable for more than several weeks even after the termination of RF. The anticipatory corticosterone peak is regarded as an expression of endogenous circadian rhythm associated with the feeding schedule. In this study, this phenomenon was used for estimating the level of learning and memory of rats. The following results was obtained.(1) The anticipatory corticosterone peak did not appear under restricted daily parenteral feeding or under restricted daily nutrition via jugular vein. These results suggested that supplement nutrition was needed for the development of the anticipatory corticosterone peak.(2) Restricted daily solid meal produced the anticipatory hormone peak in 8 days, while restricted daily liquid meal produced the peak in 12 days. The developed hormone peak was less prominent by liquid meal than by solid.(3) The anticipatory corticosterone peak developed by solid meal persisted throughout the period examined (3 weeks) after the termination of RF, while the peak developed by liquid meal disappeared after the first week.It is concluded that liquid meal attenuates the meal anticipation in the adrenocortical activity in rats under RF. These results suggested that the mastication affected the development and persistence of memory in rats.

本研究的目的是开发一种新的系统，用于估计通过成年生物学方法估算大鼠的学习水平，并检查咀嚼性是否会影响学习和记忆。在RF下，从每日饮食供应之前的2小时（预期峰）出现了8至10天的皮质酮峰，即使在RF终止后也可以检测到几周以上。预期的皮质酮峰被认为是与进食时间表相关的内源性昼夜节律的表达。在这项研究中，这种现象用于估计大鼠的学习水平和记忆水平。（1）预期性皮质酮峰未出现在日常肠胃外喂养或通过颈静脉受限的日常营养受限的情况下。这些结果表明，需要补充营养来开发预期性皮质酮峰。（2）限制的每日固体餐在8天内产生了预期的激素峰，而受限的每日液体粉则在12天内产生了峰值。（3）（3）在RF终止后（3周）中持续存在的预期皮质酮峰不如液体粉。这些结果表明，咀嚼性影响了大鼠记忆的发展和持久性。