Diagnosis of oral cancer by ^<11>C-Choline PET

^<11>C-胆碱PET诊断口腔癌

基本信息

批准号：
12671837
负责人：
SHOZUSHIMA Masanori
金额：
$ 1.92万
依托单位：
Iwate Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671837/
关键词：
^<11>C-Choline PET oral cancer cell cycle 11C-コリン口腔癌グルコースコリン癌細胞

项目摘要

Positron emission tomography using fluorine-18 fluoro-deoxyglucose (FDG PET) is useful of the detection of malignant tumor recurrences and for the evaluation of a therapeutic response. Recently, ^<11>C- Choline was introduced as another tumor tracer and successfully visualized malignant tumor. In this research, we evaluated possibility to clinical application of ^<11>C-Choline PET. The relationship between ^<11>C-choline uptake and the cell cycle phase in HeLa S3 cells, as well as how they compare to the conventional tracer FDG and ^<11>C-Methionine (Met)At first we constructed a computer -controlled apparatus of ^<11>C-Choline synthesis for routine clinical application of PET. 2-dimethylaminoethanol was loaded onto a Sep-Pak Accell CM cartridge, subsequent to the trapping of ^<11>C-methyl iodide. As a result, the average amount of ^<11>C Choline at end of synthesis was 140 mCi, the radiochemical purity was more than 99%. The simplicity of this method and the use of disposable materials for purification way be advantageous for routine clinical use. FDG uptake in HeLa S3 cells was significantly higher in the early S phase and G2/M phase compared to the G1 phase. In addition, Met and ^<11>C-Choline uptakes were higher in the G2/M phase. It has been concluded cell cycle dependency is reflected in the uptake of FDG, Met and ^<11>C-Choline seen during PET imaging of tumor tissue. These results reveal tumor proliferative activity, and can assist in evaluating a therapeutic response.Furthermore, ^<11>C-Choline was administered to a rabbit neoplasm model, and it was observed in PET, and neoplasm locus was identified precisely, and what imaging of neoplasm could apply was shown in ^<11>C-Choline-PET.

使用氟-18氟脱氧葡萄糖（FDG PET）的正电子发射断层扫描对于检测恶性肿瘤复发和评估治疗反应是有用的。最近，将 ^<11> c-胆碱作为另一个肿瘤示踪剂引入，并成功可视化了恶性肿瘤。在这项研究中，我们评估了 ^<11> c-胆碱PET的临床应用的可能性。 HeLa S3细胞中的 ^<11> c-胆碱摄取与细胞周期阶段之间的关系，以及它们如何与常规的示踪剂FDG和 ^<11> c-methionine（Met）进行比较，我们首先构建了一个计算机控制的pet量 ^<11> c-胆碱合成的设备，以实现PET的常规临床应用。将2-二甲基氨基乙醇加载到Sep-Pak Accell CM墨盒上，此后捕获 ^<11> c-甲基碘化物。结果，合成结束时 ^<11> C胆碱的平均量为140 mCi，放射性纯度超过99％。这种方法的简单性和用于纯化方式的一次性材料的使用对于常规临床使用是有利的。与G1相比，在S期，HELA S3细胞中的FDG摄取量明显更高，G2/M期明显更高。另外，在G2/m相中，MET和 ^<11> C-胆碱的摄取更高。已经得出结论，细胞周期依赖性反映在FDG，Met和 ^<11> c-胆碱的摄取过程中，在肿瘤组织的PET成像期间可见。这些结果表明肿瘤增殖活性，并可以帮助评估治疗性反应。Furthermore， ^<11> c-胆碱是针对兔肿瘤模型的，并且在PET中观察到它，并且在PET中观察到了肿瘤基因座，可以精确地鉴定出 ^11> c-Choline-pets中使用的肿瘤基因座。