The role of the nigrostriatal dopaminergic pathway in the sequential progress

黑质纹状体多巴胺能通路在顺序进展中的作用

• 批准号: 12671805
• 负责人: MASUDA Yuji
• 金额: $ 2.11万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671805/
• 关键词: microdialysis; mastication; putamen; dopamine; 6OHDA; intake behavior; 単一ニューロン活動顎運動; 顎運動; 咀嚼運動; 6-OHDA; 取り込み行動

The masticatory sequence is a whole set of movements from ingestion to swallowing. The masticatory sequence could be divided into three recognized stages (stage I, IIa, IIb) in the rabbit based on the form of jaw movements. It is, however, still unclear which neural mechanism is involved in the transition of the masticatory stages. The aim of this project was to clarify the role of the nigrostriatal dopaminergic pathway in the sequential progress during mastication.We obtained the findings in this study as follows ;1. We examined the alteration of dopamine (DA) concentration in the extracellular space of the putamen during 5 consecutive minutes of mastication in the awake rabbit, using the microdialysis. Analysis of the microdialysate samples indicated that the DA concentration in the putamen increased significantly during mastication, compared with resting the period.2. We studied the effect of bilateral 6-hydroxydopamine (6-OHDA) lesions of the nigrostriatal dopaminergic pathway on feeding behavior in freely-moving rabbits by means of video and electromyogram (EMG) of masticatory muscles. We observed that the duration of intake behavior, in which the rabbit inserted its mouth into the pellet-box and seemed to pick up pellets, was shortened after the lesions. Further, the number of intake behaviors for consuming 1 g of pellets was increased. During intake behavior, masseteric EMG activities varied in the control rabbits, whereas this variation was smaller in the lesioned subjects. This suggests that the behaviors of picking up the pellets and chewing them were intermingled during intake behavior in the control subjects. This effect was observed only when the dopaminergic pathway from the substantia nigra to the ventral part of the putamen was interrupted, and not when the dorsal part was disrupted.These results suggested that the nigrostriatal dopaminergic pathway might play a role in the sequential progress during mastication.

咀嚼序列是从摄入到吞咽的一整套动作。根据下颌运动的形式，兔子的咀嚼序列可分为三个公认的阶段（阶段I、IIa、IIb）。然而，目前尚不清楚哪种神经机制参与咀嚼阶段的转变。本项目的目的是阐明黑质纹状体多巴胺能通路在咀嚼过程中顺序进展中的作用。我们在本研究中获得如下结果；1．我们使用微透析检查了清醒兔子连续 5 分钟咀嚼过程中壳核细胞外间隙中多巴胺 (DA) 浓度的变化。微透析液样品分析表明，与静息期相比，咀嚼期壳核中DA浓度显着增加。 2．我们通过视频和咀嚼肌肌电图（EMG）研究了黑质纹状体多巴胺能通路双侧 6-羟基多巴胺（6-OHDA）损伤对自由活动兔子进食行为的影响。我们观察到，兔子将嘴插入颗粒盒并似乎拾起颗粒的摄入行为的持续时间在损伤后缩短。此外，消耗1g颗粒的摄入行为的次数也增加了。在进食行为期间，对照兔子的咬肌肌电图活动有所不同，而病变受试者的这种变化较小。这表明，对照组受试者的摄入行为中混合了捡起颗粒和咀嚼颗粒的行为。仅当从黑质到壳核腹侧部分的多巴胺能途径被中断时才能观察到这种效应，而当背侧部分被破坏时则不会观察到这种效应。这些结果表明黑质纹状体多巴胺能途径可能在咀嚼过程中的顺序进展中发挥作用。

项目成果

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