Molecular biological study on the pathophysiological substances and their interactions concerned with the enlargement of jaw cyst

颌骨囊肿增大病理生理物质及其相互作用的分子生物学研究

基本信息

批准号：
10470426
负责人：
KIMURA Hiroto
金额：
$ 4.1万
依托单位：
HIROSAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10470426/
关键词：
jaw cyst bone resortion inflanunatory cytokine cyst wall fibroblast immunohistolocal stainin messenger RNA PCR nitricoxide radical 嚢胞壁細胞

项目摘要

A few researchers have suggested that the intracystic fluid pressure and/or several bone resorbing factors might concern with the jaw cyst enlargement. Therefore, in this study, the production of inflammatory cytokines and nitric oxide (NO) in cyst fluid and/or cyst wall were investigated because we speculated these pathophysiological substances might affect the functioh of osteoblasts and/or osteoclasts located in surrounding bone of jaw cyst. Results were as follows.1. Interleukin 6 (IL-6) and tumor necrosis factor alfa (TNF-α) were detected in the cyst wall tissue and it was confirmed that the fibroblast mainly produced both cytokines.2. Using RT-PCR analysis, the expressions of messenger RNA for inflammatory cytokines (IL-6, IL-8, TNF-α, IFN-γ, TGF-β1) and the recepter of cytokines and eicosanoids (IL-6R α, gp130, TNFR I, CXCR2) were detected in supernatants of cultured fibroblast from the radicular cyst wall.3. Interleukin beta (IL-β), transforming growth factor beta 1 (TGF- β 1) and macrophage colony stimulating factor (M-CSF) were detected in the cyst fluid. NO metabolites (NOィイD22ィエD2 /NOィイD23ィエD2) were detected in the cyst fluid obtained from radicular cysts and postoperative maxillary cysts.These results suggested that such pathophysiological substances as inflammatory cytokines, eicosanoids and nitric oxide metabolites were related to the mechanism of the resorption of surrounding bone adjacent to cyst wall. These studies also revealed that the production of bone resorbing factors above described played important roles in the enlargement of jaw cyst and that these factors were derived from fibroblasts in cyst wall and were probably induced by mechanical stress.

一些研究人员提出，在这项研究中，可能会研究囊肿的炎性液体和/或几个骨骼储备因素。这些病理生理物质可能会影响骨细胞的功能，而在周围的颌骨骨骼中的骨质细胞也如下。被确认thain thain thain the the炎性细胞因子（IL-6，IL-8，TNF-α，IFN-γ，TGF-β1）的F Messenger。 I，CXCR2）是培养的fiblast的探测器。在囊肿的囊肿中，在囊肿的囊肿中检测到了囊肿的囊肿。所描述的骨骼吸收因子在下颌囊肿的扩大中起着重要作用，这些因素是YST壁，可能是由机械应力引起的。