The influence of mechanical stress on the gene expression related to the differentiation and the growth of bone cell

机械应力对骨细胞分化和生长相关基因表达的影响

• 批准号: 12470433
• 负责人: KIMURA Hiroto
• 金额: $ 8.96万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12470433/
• 关键词: Osteoblast; Mechanical stress; Cyclic tensile strain; Quantitative RT-PCR; Nitric oxide; MAP kinase; sRANKL; Osteoprotegerin; Osteoprotegerin; 定量PCR法; TAクローニング; 機械的刺激; インターロイキン6

To study the influence of physical stimulus 6n bone remodeling, using a model of mechanical stress on osteoblasts, we investigated the production of NO and the expression and/or activation of NOS, Cox-2, and MAPKs. Furthermore, the synthesis of receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) were investigated by ELISA.Results were as follows.1. Mechanical stress (cyclic tensile strain) against osteoblasts induced the NO production and the expression for iNOS mRNA.2. By the quantitative RT-PCR method, mechanical stress increased the expression for Cox-2 mRNA. But the expression of mRNA of cytokine and cytokine-receptors were not changed.3. The p38 MAPK was activated just after starting to add mechanical stress, on the contrary, ERK1/2 and JNK were not activated. Mechanical stress for 3 days, p38 MAPK activation was kept and ERK1/2 activation was down-regulated day by day.4. Adding mechanical stress at 4 hrs a day for 3days, sRANKL release was decreased and OPG synthesis was increased, simultaneously.These results suggested that p38 MAPK in osteoblasts was activated by adding mechanical stress. It was revealed consequently that mechanical stress activated osteoblasts to induce NO production, expression for Cox-2 mRNA and OPG through p38 MAPK activation. It was suggested that mechanical stress might enhance the function of osteoblasts to form new bone.

为了研究物理刺激6N骨重塑的影响，使用对成骨细胞的机械应力模型，我们研究了NO的产生以及NOS，COX-2和MAPK的表达和/或激活。此外，ELISA研究了NF-κB配体（RANKL）和骨蛋白酶蛋白（OPG）的受体活化剂的合成。分子如下。1。机械应力（环状拉伸应变）针对成骨细胞诱导NO产生和INOS mRNA的表达。2。通过定量RT-PCR方法，机械应力增加了COX-2 mRNA的表达。但是细胞因子和细胞因子受体的mRNA的表达没有改变。3。 p38 MAPK在开始增加机械应力后激活，在对比度上，ERK1/2和JNK未激活。机械应力为3天，保留P38 MAPK激活，而ERK1/2的激活日常下降。4。每天4小时增加3天的机械应力，Srankl释放增加了，很简单。这些结果表明，通过添加机械应力激活成骨细胞中的p38 MAPK。因此，揭示了机械应力激活的成骨细胞，以通过p38 MAPK激活诱导COX-2 mRNA和OPG的表达。有人提出，机械应力可能会增强成骨细胞形成新骨的功能。