The Clinical and Fundamental Study of Free Radicals Produced by Anticancer Drugs and Function of Blood Cells

抗癌药物产生的自由基与血细胞功能的临床和基础研究

• 批准号: 62570889
• 负责人: KIMURA Hiroto
• 金额: $ 1.28万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570889/
• 关键词: Free radical; Active oxygen; Anticancer drug; Bleomycin; Oral Cancer; Polymorphonuclear leukocyte; Electron Spin Resonance; Chemiluminescence; 化学発光法; 化学発行; フリーラジカル; 赤血球膜流動性; スピンラベル法

As the method to detect free radicals, I have applied the CLA - dependent chemiluminescence specific to superoxide anion radical ( O^-_ and the ESR spin trapping technique highly sensitive to hydroxyl radical ( ・OH ) by this Grant-in-Aid.1. The Effects of Anticancer Drugs on the Active Oxygen Produced by Polymorphnuclear Leukocyte(PEN). The production of O^-_ from PMN stimulated by OZ or PMA was remarkably enhanced by the addition of BLM and its analog, Peplomycin (PEP). The effect of PEP is stronger than that of BLM. Any other anticancer drugs did not influence on the generation of O^-_ from PMN. The method to measure O^-_ is CLA -dependent chemi-luminescence using luminescence reader purchased by this Grant-in-Aid.2. Clinical Study of the Active Oxygen Production by PMN from Oral Cancer Patients. The O^-_ production ability of PMN obtained from patients showed low values compared to that of PMN from healthy volunteers. The administration of anticancer drugs to the patients had no effect on the O^-_ production ability of PMN from the patients except for PMA as a stimulant. These results suggested that the primary host-defence mechanism of patients were depressed. The investigation of the changes for long periods must be the subject of further study.3. Effect of Anticancer Drugs on Free Radical Generation Systems in vitro. I report here the effect of BLM and PEP on free radical generation systems, which were (1)HX+XOD -> O^-_ and (2)Fe(II)+H_2O_2-> ・OH, using chemiluminescence method and ESR spin trapping technique. As results, both BLM and PEP increased the free radical generation in proportion to the concentration of drugs. The effect of PEP is two times stronger than that of BLM same as described above.These results suggested that the Fe(II) - BLM complex reacted with free radicals to produce secondarily hydroxyl radicals.

作为检测自由基的方法，我已经对超氧化物阴离子的特异性化学发光施加了对羟基自由基的诱捕技术。氧气多核白血病（PEN）通过添加BLM及其类似物，PEPLOMYCIN（PEP）明显增强。与健康志愿者的PMN相比，使用该赠款的含量为2。对于患者的O^ - __生产能力没有影响。 3。iCal Generation Systems，是（1）HX+XOD-> o^-_和（2）Fe（II）+H_2O_2-> oh，使用化学发光方法自旋陷阱技术。 PEP的效果是BLM的两倍，与Desults相同，表明Fe（II）Darily羟基自由基。

项目成果

木村博人: "口腔癌患者末梢血多形核白血球のス-パ-オキサイド産生について" 日本口腔科学会誌. 39. (1990)

Hiroto Kimura：“口腔癌患者外周血多形核白细胞中的超氧化物产生”，日本口腔医学会杂志 39。（1990）。

木村博人,伊藤浩昭,鈴木貢 他: 日本口腔科学会誌. 37. (1988)

Hiroto Kimura、Hiroaki Ito、Mitsugu Suzuki 等：日本口腔医学会杂志 37。（1988）

KIMURA, Hiroto: "Superoxide Production of Polymorphonuclear Leukocyte from Oral Cancer Patients" J. Jpn. Stomatol. Soc., vol. 39, 1990.

KIMURA, Hiroto：“口腔癌患者多形核白细胞的超氧化物产生”J. Jpn。

木村 博人: 日本口腔科学会誌. 38. (1989)

木村博人：日本口腔医学会杂志 38。（1989）

KIMURA, Hiroto: "Effects of Bleomycin and Peplomycin on Free Radical Generation Systems" J. Jpn. Stomatol. Soc., vol. 39, 1990.

KIMURA, Hiroto：“博莱霉素和培洛霉素对自由基生成系统的影响”J. Jpn。