Study on the cooperative bactericidal activities between neutrophis and antibiotics, electrolyzed-oxidizing water in serious infection disease

中性粒细胞与抗生素、电解氧化水在严重感染性疾病中协同杀菌作用的研究

基本信息

批准号：
07457482
负责人：
KIMURA Hiroto
金额：
$ 2.18万
依托单位：
HIROSAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

O_2^<・->production abnormalities of neutrophils in peripheral blood with severely infectious disease in maxillofacial region were investigated by CLA dependent chemiluminescence method. The effect of antibiotics on O_2^<・->production of neutrophils was also studied. Furthermore, bactericidal effects of electrolyzed-oxidizing water on Stapylococcus aureus (S.aureus) were investigated by O_2^<・->production of neutrophils and expression of integrin molecules.Results were as follows.1. O_2^<・->production of neutrophils obtained from 12 patients with oral cancer and 6 patients with mandublar osteomyelitis showed lower values than heal thy volunteers. Six of 12 patients with oral cancer were suffered from postoperative infection by MRSA.2. Four antibiotics (ASPC,NTL,ISP,FLRX) increased O_2^<・->production of neutrophils to show cooperative bactericidal activities. And three of them (ASPC,NTL,FLRX) increased the expression of C3b recepter on the suface of neutrophil membrane.13EA04 : 3. O_2^<・->production of neutrophils accompanying with phagocytesis of S.aureaus (1*10^8/ml) , which were pretreated by electrolyzed-oxidizing water, showed 50% of bacteria was injured by this treatment. The expression of integrin molecules' (CD11b and CD18) on the surface of neutrophil membrane with phagocytosing S.aureus revealed that 70-80% of bacteria lost their biological activities by the treatment with electrolyzed-oxidizing water.4. Surgical wound infected by MRSA was remarkably improved by washing with electrolyzed-oxidizing water. In treating MRSA infection patients, optimal dosage of Vancomycin^<<encircledR>>was determined by monitoring its concentration in peripheral blood.These results suggested that it was useful to choose antibiotics based on the cooperative activity which increased O_2^<・->production of neutrophils. Electrolyzed-oxidizing water showed fatal effects on peccant bacteria, therefore, its clinical use was more recommended.

O_2^<・->在颌面cla依赖性化学发光法中，外周血腥疾病的产生异常。 O_2^<rin分子研究了s. aureus。拟人分子。1 MRSA的术后感染2。 Membrane.13EA04：3。O_2^<.->中性粒细胞的产生，伴随着S.aaureas的吞噬作用（1*10^8/ml），它们是预处理的氧化水。）在中性粒细胞膜的表面上，吞噬的s. aureus表明，他们的蜜蜂作用是由MRSA感染的手术性伤口。万古霉素的剂量是通过监测其在外周血中的contration依的剂量。此类剂量表明，基于相结合的活性，选择抗生素是有用的因此，在特性细菌上，更建议其临床用途。