Study on the pathological absorption of maxillary bone induced by mechanical stress

机械应力引起上颌骨病理吸收的研究

• 批准号: 05454537
• 负责人: KIMURA Hiroto
• 金额: $ 3.65万
• 依托单位: HIROSAKI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454537/
• 关键词: jaw cyst; bone absorption; mechanical stress; intracystic pressure; microcirculation; lipid peroxide; antioxidant enzyme; cytokine; 微小循環障害; 骨吸収因子; レーザードップラー血流計; シッフ塩基染色法

To solve the relationship between the physical force ( =mechanical stress)and the bone absorption in jaw cysts, we tried to measure directly intracystic pressure and colloid osmotic pressure of cyst fluids. The cyst wall microcirculation was also measured by laser doppler blood flow meter. Using cyst wall tissues as materials, we tried to detect lipid peroxide by chemiluminescence-HPLC method, prostanoid and inflammatory cytokines by ELISA.Furthermore, the localization of lipid peroxide and antioxidant enzyme in cyst wall were investigated by the histochemical method. Results were as follows.1. The average of intracystic pressure was 27.4mmHg, which was a good correlation with the colloid osmotic pressure, and changed with the degeneration of cyst fluid accompanying with bacterial infection or inflammation. No correlation could be found between the pressure and the histological typing of jaw cysts. 2. The blood flow of cyst wall showed lower value than healthy-side gingival mucosa. 3. … More PGE_2, IL-1betaIL-6, TNF alpha were detected in cyst wall. Values of these factors were in propotion to the degree of inflammation in jaw cysts. 4.Cu, Zn-SOD was localized at the inner side of cyst wall epitelium and at the capillary endothelial epithelium in cyst wall connective tissue. This finding was in good accordance with both pathohistological finding of inflammation and the localization of lipid peroxide staining in jaw cyst wall.These results in this study revealed that the increase of intracystic pressure was induced by the free radicals-mediated tissue injury and that the microcirculation in cyst wall was also damaged by the increasing of the colloid osmotic pressure of cyst fluid. These findings strongly suggested that the microvascular injury was responsible for the generation of bone absorbing factor. In clinical study, jaw cyst gradually decreased in size according to the declines of intracystic pressure by the irrigation therapy, which aspirate cyst fluid regularly to change physiological saline including antioxidant reagents. Less

为了解决颌骨囊肿中物理力（=机械应力）与骨吸收之间的关系，我们尝试直接测量囊肿液的囊内压和胶体渗透压，并通过激光多普勒血流计测量囊肿壁微循环。以囊壁组织为材料，尝试采用化学发光-HPLC法检测脂质过氧化物，采用ELISA法检测前列腺素和炎性细胞因子。采用组织化学方法检测囊壁抗氧化酶，结果显示囊内压平均值为27.4mmHg，与胶体渗透压有良好的相关性，并随着囊液伴细菌的变性而变化。压力与颌骨囊肿的组织学分型之间没有发现相关性。 2. 囊肿壁的血流值低于健侧牙龈粘膜。 3. … 更多囊壁中检测到PGE_2、IL-1β、IL-6、TNFα，这些因子的值与囊肿颌的炎症程度成正比。4.Cu、Zn-SOD定位于囊壁内侧。囊肿壁结缔组织中的上皮和毛细血管内皮上皮，这一发现与炎症的病理组织学发现和囊肿颌壁中的脂质过氧化物染色的定位非常一致。本研究结果表明，强自由基介导的组织损伤导致囊内压升高，囊液胶体渗透压升高也导致囊壁微循环受损。临床研究中，颌骨囊肿随着囊内压力的下降而逐渐缩小，冲洗疗法定期抽吸囊液以更换含有抗氧化剂的生理盐水。较少的

项目成果

小川 透: "顎嚢胞壁の脂質過酸化と微小循環動態の障害について" 過酸化脂質研究. 18(印刷中). (1995)

Toru Okawa：“颌囊肿壁中的脂质过氧化和微循环动力学的紊乱”脂质过氧化物研究18（出版中）。

秋田谷 徹,木村博人他: "顎嚢胞性疾患におけるCu,Zn-SODの局在に関する免疫組織化学的研究" 過酸化脂質研究. 18(印刷中). (1995)

Toru Akitaya、Hiroto Kimura 等：“囊性颌病中 Cu,Zn-SOD 定位的免疫组织化学研究”脂质过氧化物研究（1995 年）。

Hiroto KIMURA,Yositaka TAKACHI: "The role of prostaglandins and cytokines in bone absorption." The Journal of Dental Medicine. 39-4. 387-393 (1994)

Hiroto KIMURA、Yositaka TAKACHI：“前列腺素和细胞因子在骨吸收中的作用。”

Tohru AKITAYA,Hiroto KIMURA et al.: "Immunohistochemical study on the localization of Cu, Zn-SOD in the tissue of jaw cysts." Lipid Peroxide Research. 18-3, (in press, ). (1995)

Tohru AKITAYA、Hiroto KIMURA 等人：“颌骨囊肿组织中 Cu、Zn-SOD 定位的免疫组织化学研究”。

佐藤 寿: "CL-HPLC法による顎嚢胞壁組織の過酸化脂質の測定" 過酸化脂質研究. 18. 277-280 (1995)

Hisashi Sato：“通过 CL-HPLC 方法测量颌囊肿壁组织中的脂质过氧化物”脂质过氧化物研究 18. 277-280 (1995)。