Identification of biological factors involving in serial culturing technique for germ cell series

生殖细胞系列连续培养技术涉及的生物因子的鉴定

• 批准号: 10306018
• 负责人: SATO Eimei
• 金额: $ 22.78万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10306018/
• 关键词: hyaluronic acid; MAP kinase; early embryogenesis; sex chromosome; oocyte maturation; in vitro fertilization; germ cells; embryo-derived cell; 卵子; 胚の凍結保存; サイクリンB; cdc25; 甲状腺ホルモン; 卵丘膨化; マウス; ブタ; 単為発生; 卵形成; 卵成熟; Primordial germ cells(PGC)

1) Mitogen-activated protein kinase (MAPK) mediates the maturation-inducing signal from the cytoplasm into nucleus and induces meiosis reinitiation in porcine oocytes.2) Two isoforms of cDNA encoding novel zinc finger proteins which play important roles in the development of the testes was cloned.3) Both microtubules and microfilaments are involved in the developmental block in two-cell rat embryos and the developmental block in rat two-cell embryos is induced by disturrbance of mitochondrial energy metabolism. MAP kinase cascade is activated at the M-phase and some MAP kinases other than ERKs are activated during early cleavage of mouse embryos.4) Rapid detection of male-specific DNA sequence in bovine embryos using FISH method was established.5) Gonadotropin-inducible NAIP indirectly affect oocyte survival as a result of the inhibition of apoptotic granulosa-cell death during folliculogenesis.6) The effect of hyaluronic acid on the development of in vitro-produced porcine embryos varies with the condition of oocyte maturation and fertilization.7) A porcine cell line can be established from the hatched blastocyst and maintained in vitro for a long period, and that reconstructed embryos obtained by transferring the blastocyst-derived cells into enucleated oocytes have the ability to develop to the blastocyst stage in vitro.

1）有丝分裂原激活的蛋白激酶（MAPK）介导了细胞质的成熟诱导信号到核中，并诱导猪卵母细胞中的减数分裂重新入射。2）编码新型锌指蛋白质的cDNA，这些cDNA在新型的锌指蛋白中起发育的重要作用3）三细胞大鼠胚胎中的发育块中均参与微管和微丝，并且大鼠两细胞胚胎的发育阻滞均由线粒体能量代谢的蒸馏而诱导。在小鼠胚胎的早期切割过程中激活了MAP激酶级联反应，而ERKS以外的其他MAP激酶则被激活。4）使用FISH方法在牛胚中快速检测雄性特异性DNA序列。5）5）5）促性腺素诱导蛋白诱导由于卵泡发生过程中抑制凋亡颗粒细胞死亡的抑制作用。6）透明质酸对体外产生的猪胚胎的发展而变化，随着卵母细胞成熟和肥料的变化。7）可以从孵化的胚泡中建立猪细胞系并长时间维持体外，并通过将胚泡衍生的细胞转移到蛋白核细胞中获得的重建胚胎可以在体外在体外发展为胚泡阶段。

项目成果

Eimei Sato: "Reproductive Biology in Mammals"Gakusousha. 1-300 (1999)

佐藤英明：“哺乳动物的生殖生物学”Gakusousha。

佐藤英明: "Asparagine-linked glycosylation of the rat leukemia inhibitory factor expressed by Simian COS7 cells" Bioscience,Biotechnology and Biochemistry. 62. 1318-1325 (1998)

Hideaki Sato：“猿 COS7 细胞表达的大鼠白血病抑制因子的天冬酰胺连接糖基化”《生物科学、生物技术和生物化学》62. 1318-1325 (1998)。

佐藤英明: "Evaluation of fluid from cystic follicles for in vitro maturation and fertilization of bovine oocytes" Theriogenology. 50. 307-320 (1998)

Hideaki Sato：“牛卵母细胞体外成熟和受精的囊泡液体评估”Theriogenology。50. 307-320 (1998)。

佐藤英明: "ウシ受精卵の性判別のための迅速FISH法"日本哺乳動物卵子学会誌. 16. 77-81 (1999)

Hideaki Sato：“快速 FISH 方法确定牛受精卵的性别”日本哺乳动物卵学会杂志 16. 77-81 (1999)。

梅津元昭: "Evaluation and characterization of Congenital hypothyroidism in row dwarf rats" Laboratory Animal Science. 48. 496-501 (1998)

Motoaki Umezu：“行侏儒大鼠先天性甲状腺功能减退症的评估和特征”实验动物科学 48. 496-501 (1998)。