Improvement of IVM based on the elucidation of meiosis pause mechanism by uppermost stream factor identification of oocyte maturation inhibitory factor group

基于卵母细胞成熟抑制因子群最上游因子鉴定阐明减数分裂暂停机制的IVM改进

• 批准号: 23658236
• 负责人: SATO Eimei
• 金额: $ 2.5万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658236/
• 关键词: 卵成熟; 卵成熟抑制因子; 減数分裂休止; IVM; ＩＶＭ; 卵子; 卵成熟抑制; ｃ型ナトリウム利尿ペプチド; IVM法; Akt; 体外成熟卵子

We tried the elucidation for the global image of the meiosis pause mechanism seen in oocyte maturation process based on the previous results of research. The upstream factor was searched based on the data of a DNA microarray, and it was shown clearly that C-Type natriuretic peptide and adrenomedullin lead to identification of an upstream factor. Furthermore, we also tackled improvement of IVM and it was shown clearly that fertilizing capacity and the developmental competence after fertilization go up by in vitro oocyte maturation for a definite period of time by cAMP or a cAMP potentiation factor.

我们尝试根据以往的研究成果，对卵母细胞成熟过程中减数分裂暂停机制的整体图像进行阐明。基于DNA微阵列的数据搜索上游因子，清楚地表明C型利尿钠肽和肾上腺髓质素导致上游因子的鉴定。此外，我们还解决了IVM的改进问题，清楚地表明，通过cAMP或cAMP增强因子在一定时间内体外卵母细胞成熟，受精能力和受精后的发育能力得到提高。

项目成果

Adrenomedullin: A possible regulator.

肾上腺髓质素：一种可能的调节剂。

• 发表时间: 2011
• 期刊: Reprod.Biotechnol
• 作者: Hiradate,Y.;Tanemura,K.;Sato,E
• 通讯作者: Sato,E

マウス卵母細胞減数分裂再開抑制におるアドレノメジュリンの作用

肾上腺髓质素对抑制小鼠卵母细胞减数分裂恢复的作用

• 发表时间: 2012
• 作者: 平舘裕希;大竹順;星野由美;種村健太郎;佐藤英明
• 通讯作者: 佐藤英明

Distribution and Y397 phosphorylation

分布和 Y397 磷酸化

• 发表时间: 2011
• 期刊: Cell Tissue Res
• 影响因子: 3.6
• 作者: Sakurai,M.;Sato,E
• 通讯作者: Sato,E

Factors affecting meiotic arrest in mammalian oocytes

影响哺乳动物卵母细胞减数分裂停滞的因素

• 发表时间: 2012
• 作者: Sakurai,M.;Sato,E;Sato E
• 通讯作者: Sato E

Adrenomedullin: A possible regulator of germinal vesicle breakdown

• DOI: 10.1016/j.bbrc.2011.10.139
• 发表时间: 2011-12-02
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Hiradate, Yuuki;Ohtake, Jun;Sato, Eimei
• 通讯作者: Sato, Eimei