Identification of genes and angogenic factors involving in selective growth and apoptosis of oocytes

鉴定参与卵母细胞选择性生长和凋亡的基因和血管生成因子

• 批准号: 13460129
• 负责人: SATO Eimei
• 金额: $ 7.42万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-13460129/
• 关键词: Oogenesis; atresia; angiogenesis; HAS; CD44; thyroxine; VEGF; VEGF gene fragment; 卵子形成; TNF-α; bFGF; KLt-1; FLk; KDR; TNF-a; Klt-1; Flk; 卵胞発育; 血管網; FLK-1; FLT-1; 排卵; ブタ

1) It was clarified that Hyaluronic acid-binding proteins expressed in porcine COCs during IVM, particularly CD44, may form a network of the matrices in the extracellular space of the oocyte with cumulus expansion during INM.2) Infertile rdw rates responded well to repeated stimulation with gonadotropins, and T4 treatment increased the total number of ovulated eggs in an individual rat treated three times with gonadotropins.3) Porcine oocyte promote eCG-dependent cumulus expansion and the expression of has2 mRNA in cumulus cells, but these are not essential for expansion of cumulus cells and the expression of has2 mRNA. HAS2 is involved in HA synthesis during cumulus expansion, and eCG and pFF up-regulate its expression. The expresion profile of the has 3 mRNA that is transcribed in oocytes is different from those of has 2 and CD44 mRNA. CD44 may aprticipate in the interaction between cumulus cells and oocytes.4) Follicular vascular baskets of diverse sizes and architecture in various developmental stages support the gradua lincrease of follicular blood flow during follicule growth in the pig.5) We demonstrated that angiogenesis increases during bovine follicular growth and occurs uneven in different inner theca regions of the follicles.6) We offered an innovative technique for enhanced induction of follicular development in the ovary through gene and hormonal treatment, which may lead to prevention of infertility caused by ovarian dysfunction.7) A technique for direct ovarian injection of GDF-9 gene fragment was developed. It may contribute to novel therapy for prevention and treatment of infertility associated with ovarian dysfunction.

1）澄清说，IVM期间在猪COC中表达的透明质酸结合蛋白（尤其是CD44）可能形成一个在卵母细胞外卵母细胞外的矩阵网络，并在inmmmtile Rdw速率上响应良好，以响应良好用促性腺激素刺激和T4治疗增加了用促性腺激素处理三次的个体大鼠的排卵卵总数。3）猪卵母细胞促进ECG依赖性库卢斯的膨胀和库卢斯细胞中HAS2 mRNA的表达，但这些对于这些细胞中并不是必需的积云细胞的膨胀和Has2 mRNA的表达。 HAS2参与了积云扩张期间的HA合成，ECG和PFF上调了其表达。在卵母细胞中转录的3个mRNA的排列曲线与2和CD44 mRNA的分数不同。 CD44 5月5月在卵形细胞与卵母细胞之间的相互作用中。4）在各种发育阶段，各种大小和建筑的卵泡血管篮有助于猪的卵泡血流的Gradua lincrease follicular lincrease follicular flow follicule the Pig.5）我们证明了在牛dy虫中的毛囊血液流量。卵泡生长在卵泡的不同内部theca区域不均匀。6）我们提供了一种创新的技术，可以通过基因和激素治疗增强卵巢中卵泡发育的诱导，这可能导致预防由卵巢功能障碍引起的不孕症。7）7）开发了一种直接卵巢注射GDF-9基因片段的技术。它可能有助于预防和治疗与卵巢功能障碍相关的不孕症的新疗法。

项目成果

佐藤英明: "Follicular microvasculature and angiogenic factors in the ovaries of domestic animals"J.Reprod.Dev.. 49・3. 181-192 (2003)

佐藤英明：“家畜卵巢中的卵泡微血管和血管生成因子”J.Reprod.Dev.. 49・3（2003）。

Biolog of porcine cumulus-oocyte complexes ; Recent advances and possible significance on animal production

猪卵丘-卵母细胞复合物的生物学；

• 发表时间: 2003
• 期刊: Animal Frontier Sciences-Life science update in animal science
• 作者: 清水 隆;江 金益;飯島康二;宮林香奈子;小川好則;佐々田比呂志;佐藤英明;Eimei Sato
• 通讯作者: Eimei Sato

佐藤英明: "血管増殖促進因子を用いる新しい排卵誘発法"化学と生物. 41・4. 225-231 (2003)

佐藤英明：“利用血管生长促进因子的新排卵诱导方法”化学与生物学41・4（2003）。

佐藤英明: "血管新生因子と卵子"Hormone Frontier in Gynecology. 11・1. 36-45 (2004)

佐藤秀明：“血管生成因子和鸡蛋”妇科激素前沿 11・1（2004 年）。

Animal Technology

动物技术

• 发表时间: 2004
• 作者: Takashi Shimizu;Jin-Yi Jiang;Koji Iijima;Kanako Miyabayashi;Yoshinori Ogawa;Hiroshi Sasada;Eimei Sato;佐藤英明;Eimei Sato
• 通讯作者: Eimei Sato